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Figure 2 | BMC Immunology

Figure 2

From: Respiratory burst function of ovine neutrophils

Figure 2

A comparison of the effects on PMA-induced ovine neutrophil respiratory burst function when varying the length of PAF incubation, and the order of agonist addition. Ovine neutrophils were incubated with either a single agonist (PAF or PMA; n = 27 and n = 26 respectively) or with a combination of PAF and PMA. Incubation times investigated were 0 minutes (simultaneous addition of PAF and PMA; n = 19), and both 5 and 10 minutes (PAF followed by PMA; n = 18 and n = 21 respectively). The order of addition was also varied, with PMA being added first, incubated for 10 minutes, and then PAF being added (n = 8). Results are shown for V max (A), time taken to reach V max (B), the total amount of O2- released (C), and reaction duration (D). Results are shown in the form of a Box and Whiskers graph, and groups were compared using one way ANOVA followed by post hoc Bonferroni test for multiple comparisons. Significance was determined at P < 0.05.

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