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Table 1 Characteristics of ovine neutrophil respiratory burst function in response to a range of priming and activating agonists

From: Respiratory burst function of ovine neutrophils

 

n

 

V max

(nmoles O2-/2 × 105 neutrophils/min)

time to V max

(min)

total amount of O2- released

(nmoles O2-/2 × 105 neutrophils)

duration of reaction

(min)

Nil

29

mean

0.216

16.0

1.885

65.5***

  

std dev

0.113

6.9

1.527

33.8

PAF

(20 μM)

28

mean

0.558

11.3**

2.657

27.5

  

std dev

0.308

23.3

1.956

21.0

TNF-α

(10 ng/mL)

6

mean

0.339

5.3

1.255

72.5

  

std dev

0.212

7.2

0.484

38.2

LPS

(50 ng/mL)

6

mean

0.650

8.7

1.106

44.2

  

std dev

0.362

5.8

0.441

32.3

PMA

(20 ng/mL)

26

mean

1.221***

14.4

24.340***

58.7

  

std dev

0.532

7.3

4.264

20.5

fMLP

(20 μM)

28

mean

0.567

5.2***

1.688

36.4**

  

std dev

0.288

5.2

1.452

31.5

  1. Ovine neutrophils (2 × 105) were incubated either with buffer only (Nil) or one of a range of priming (PAF, TNF-α, LPS) and activating agents (PMA, fMLP). Total assay length was 120 minutes. Parameters were measured as described in Methods. Asterisks represent level of significance compared to unstimulated cells (Nil); * p < 0.05; ** p < 0.01; *** p < 0.001.