Skip to main content
Figure 2 | BMC Immunology

Figure 2

From: A kinase-dead knock-in mutation in mTOR leads to early embryonic lethality and is dispensable for the immune system in heterozygous mice

Figure 2

mTOR kinase-dead allele and its confirmation in mice. (A) Nucleotide and corresponding amino acid sequence of the wild type or mutated allele. (B) PCR-based genotyping using genomic DNA and a primer set (p13 and p25) flanking the remaining loxP site. The 332 bp band corresponds to the wild type allele, while 405 bp band corresponds to the mutant allele containing the remaining loxP site.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us