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Figure 4 | BMC Immunology

Figure 4

From: A kinase-dead knock-in mutation in mTOR leads to early embryonic lethality and is dispensable for the immune system in heterozygous mice

Figure 4

Expression of mTOR in wild type and +/kd knock-in mice. (A) RT-PCR analysis of mRNA from hearts of wild-type and mTOR+/kd mice using two different pairs of primers frto1 and frto2. β-actin is used as a positive control. (B) Immunoblot of mTOR in protein extracts (10 μg) from heart tissues of mTOR+/+ and mTOR+/kd mice and its semiquantification by densitometry analysis. β-tubulin is loading control. Two independent pairs of +/+ and +/kd mice are shown.

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BMC Immunology

ISSN: 1471-2172

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