Figure 2

CAPE did not inhibit costimulatory molecule (such as CD86, CD80, and CD83) expression on MoDCs. Immature MoDCs from healthy subjects were cultured for 48 hours either in the absence or presence of CAPE (10 μM) under LPS (100 ng/mL) or crude mite extract (100 μg/mL) stimulation. Surface markers were analyzed by flow cytometry. (A) Histogram of fluorescence intensity of HLA-DR, CD86, CD80 and CD83. The values shown in the flow cytometry profiles are the mean fluorescence intensity (MFI) indexes. (B) MFI of HLA-DR, CD86, CD80, and CD83. Data shown represent mean ± SEM from three separate experiments. *P < 0.05; **P < 0.01.