Figure 1From: Sequence determinants of innate immune activation by short interfering RNAsOptimization of assay for detection of innate immune stimulation of PBMCs by siRNA. Supernatant from PBMCs treated with DOTAP complexed siRNAs for 24 hours was applied to Huh7-Luc cells. Luciferase expression was assayed 24 hours after treatment with supernatant. Each point represents the mean of triplicate samples. Error bars indicate standard deviation. (A) The indicated densities (cells/100 μL) of PBMCs were stimulated with 40 nM indicated siRNAs. (B)-(E) Dose response curves performed using PBMCs at a density of 50,000/100 μL in the presence or absence of chloroquine at the indicated concentrations.Back to article page