Enrichment and analysis of secretory lysosomes from lymphocyte populations

BMC Immunology

Table 2 Total protein and protein concentrations in individual fractions obtained after differential and density centrifugation.

fraction	Exp. 1	Exp. 2	Exp. 3	Exp. 4	mean	SD	Volume	protein
	[mg/ml]	[mg/ml]	[mg/ml]	[mg/ml]	[mg/ml]		[μl]	[μg]
homogenate	3.891	3.222	3.532	3.684	3.582	0.282	2700	9671.4
enriched organelles (input)	0.745	0.651	0.729	0.865	0.747	0.088	1800	1345.3
fraction 1	0.349	0.303	0.347	0.296	0.324	0.028	200	64.7
fraction 2	0.430	0.468	0.306	0.389	0.398	0.069	400	159.3
fraction 3	0.764	0.909	1.004	0.879	0.889	0.099	300	266.7
fraction 4	0.526	0.548	0.480	0.405	0.490	0.063	300	147.0
fraction 5	0.925	0.882	0.959	0.852	0.905	0.047	200	180.9
fraction 6	0.384	0.431	0.352	0.349	0.379	0.038	200	75.8
recovery								894.4

ISSN: 1471-2172