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Figure 3 | BMC Immunology

Figure 3

From: Interleukin-21 induces the differentiation of human umbilical cord blood CD34-lineage- cells into pseudomature lytic NK cells

Figure 3

Phenotypic features of cytokine-differentiated CD34-lineage- cells. After 4 weeks of culture with either IL-15 or the combination of IL-15 and IL-21, CD34-lineage- cells were harvested, stained with a panel of mAb directed against informative differentiation/maturation antigens and used for flow cytometry analysis. Fluorochrome-conjugated, isotype-matched mAb from the same manufacturers were used to measure background fluorescence. Mononuclear cells (MNC) from UCB and peripheral blood (PB) samples as well as NK cells matured in vitro with Flt3-L, SCF, IL-15 and IL-21 from UCB CD34+ cells (CD34+-NK15+21) were used as control for antigen expression. The percentage of cells staining positively for each given antigen is indicated in the dot plots. One representative experiment out of 8 with similar results is shown.

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