Table 3 Primers used to detect gene expression by qPCR in cytokine-differentiated CD34-lineage- cells.
Primer | Sequence (5'-3') | Orientation |
|---|---|---|
IDH2 | ACTACATCAGGGACCTTCAG | F |
IDH2 | TATAAGGATGATCTAGTGGTCG | R |
BCL-2 | AGGAAACTTGACAGAGGATCATGC | F |
BCL-2 | CGGATCTTTATTTCATGAGGCACG | R |
GATA-3 | AGGAGGAATGCCAATGGGGAC | F |
GATA-3 | TCGGTTTCTGGTCTGGATGCC | R |
CD34 | TCTTGACAACAACGGTACTGCTAC | F |
CD34 | GCTGGTACTTCCAAGGGTACTAGG | R |
GAPDH | CCTGACCTGCCGTCTAGAAA | F |
GAPDH | CTCAGTGTAGCCCAGGATGC | R |