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Table 1 Oligonucleotide primers used in this study and the expected PCR product size.

From: Characterization of rag 1 mutant zebrafish leukocytes

Gene/Fragment

Forward Primer

Reverse Primer

Size (bp)

TCR V(D) JC complete transcripts from [16]

   Vb1.5/17.5-Cb1 (first round)

aatggacagcttgatagaactgaac

aagatgacaaggccatacagtc

780

   Vb1.5/17.5-Cb1 (second round)

tgcttattcaaccgaacagaaacattc

gtccgctcttagcaatggtc

730

   Vb12-Cb1 (first round)

cagacaccgtgcttcagtcgag

aagatgacaaggccatacagtc

770

   Vb12-Cb1 (second round)

acgtttcatggcagtgttacctg

gtccgctcttagcaatggtc

720

   Vb14.5-Cb1 (first round)

gaatccaatgtgacgttaacatgc

aagatgacaaggccatacagtc

750

   Vb14.5-Cb1 (second round)

catgatcataaggaccactacag

gtccgctcttagcaatggtc

700

   Vb1.5/17.5-Cb2 (first round)

aatggacagcttgatagaactgaac

tgatctccgttgaagaatcggac

550

   Vb1.5/17.5-Cb2 (second round)

tgcttattcaaccgaacagaaacattc

gtgcaggtgaatttattgttgggatc

500

   Vb12-Cb2 (first round)

cagacaccgtgcttcagtcgag

tgatctccgttgaagaatcggac

550

   Vb12-Cb2 (second round)

acgtttcatggcagtgttacctg

gtgcaggtgaatttattgttgggatc

500

   Vb14.5-Cb2 (first round)

gaatccaatgtgacgttaacatgc

tgatctccgttgaagaatcggac

550

   Vb14.5-Cb2 (second round)

catgatcataaggaccactacag

gtgcaggtgaatttattgttgggatc

500

Ig (VDJC) complete transcripts from [16]

   igVH1-Cm (first round)

gatggacgtgttacaatttgge

acatgaaggttgctgatccac

810

   igVH1-Cm (second round)

cctcctcagactctgtggtgae

ttgctgatccaccttctaattc

740

   igVH2-Cm (first round)

ttgtaacatgaccatgaatatte

acatgaaggttgctgatccac

820

   igVH2-Cm (second round)

cgattagatcagtcaccttcte

ttgctgatccaccttctaattc

750

   igVH3-Cm (first round)

catgacaatggatattgtgtcce

acatgaaggttgctgatccac

810

   igVH3-Cm (second round)

ctctgttggtgtcaaacactge

ttgctgatccaccttctaattc

760

   igVH4-Cm (first round)

caagatgaagaatgctctctge

acatgaaggttgctgatccac

810

   igVH4-Cm (second round)

tgtcaaagtatggagtcgae

ttgctgatccaccttctaattc

760

Zebrafish NK cell receptors (primers designed for this study)

   NK cell lysin 1

ccagctaaagcaaaaccc

ggaaaggtgaaacggaag

293

   NK cell lysin 2,3

ttcattcatgttgggcgtgaca

ttgatttcatctggcgttgag

450

   NCCR

ggaagctggcagatcacaaca

acggtgtcccaatgcct

268

   EF1-α

atggcacggtgacaacatgct

ccacattaccacgacggatg

392