Gene/Fragment | Forward Primer | Reverse Primer | Size (bp) |
---|---|---|---|
TCR V(D) JC complete transcripts from [16] | |||
   Vb1.5/17.5-Cb1 (first round) | aatggacagcttgatagaactgaac | aagatgacaaggccatacagtc | 780 |
   Vb1.5/17.5-Cb1 (second round) | tgcttattcaaccgaacagaaacattc | gtccgctcttagcaatggtc | 730 |
   Vb12-Cb1 (first round) | cagacaccgtgcttcagtcgag | aagatgacaaggccatacagtc | 770 |
   Vb12-Cb1 (second round) | acgtttcatggcagtgttacctg | gtccgctcttagcaatggtc | 720 |
   Vb14.5-Cb1 (first round) | gaatccaatgtgacgttaacatgc | aagatgacaaggccatacagtc | 750 |
   Vb14.5-Cb1 (second round) | catgatcataaggaccactacag | gtccgctcttagcaatggtc | 700 |
   Vb1.5/17.5-Cb2 (first round) | aatggacagcttgatagaactgaac | tgatctccgttgaagaatcggac | 550 |
   Vb1.5/17.5-Cb2 (second round) | tgcttattcaaccgaacagaaacattc | gtgcaggtgaatttattgttgggatc | 500 |
   Vb12-Cb2 (first round) | cagacaccgtgcttcagtcgag | tgatctccgttgaagaatcggac | 550 |
   Vb12-Cb2 (second round) | acgtttcatggcagtgttacctg | gtgcaggtgaatttattgttgggatc | 500 |
   Vb14.5-Cb2 (first round) | gaatccaatgtgacgttaacatgc | tgatctccgttgaagaatcggac | 550 |
   Vb14.5-Cb2 (second round) | catgatcataaggaccactacag | gtgcaggtgaatttattgttgggatc | 500 |
Ig (VDJC) complete transcripts from [16] | |||
   igVH1-Cm (first round) | gatggacgtgttacaatttgge | acatgaaggttgctgatccac | 810 |
   igVH1-Cm (second round) | cctcctcagactctgtggtgae | ttgctgatccaccttctaattc | 740 |
   igVH2-Cm (first round) | ttgtaacatgaccatgaatatte | acatgaaggttgctgatccac | 820 |
   igVH2-Cm (second round) | cgattagatcagtcaccttcte | ttgctgatccaccttctaattc | 750 |
   igVH3-Cm (first round) | catgacaatggatattgtgtcce | acatgaaggttgctgatccac | 810 |
   igVH3-Cm (second round) | ctctgttggtgtcaaacactge | ttgctgatccaccttctaattc | 760 |
   igVH4-Cm (first round) | caagatgaagaatgctctctge | acatgaaggttgctgatccac | 810 |
   igVH4-Cm (second round) | tgtcaaagtatggagtcgae | ttgctgatccaccttctaattc | 760 |
Zebrafish NK cell receptors (primers designed for this study) | |||
   NK cell lysin 1 | ccagctaaagcaaaaccc | ggaaaggtgaaacggaag | 293 |
   NK cell lysin 2,3 | ttcattcatgttgggcgtgaca | ttgatttcatctggcgttgag | 450 |
   NCCR | ggaagctggcagatcacaaca | acggtgtcccaatgcct | 268 |
   EF1-α | atggcacggtgacaacatgct | ccacattaccacgacggatg | 392 |