- Research article
- Open Access
Tumor infiltrating lymphocytes: an intriguing player in the survival of colorectal cancer patients
© Deschoolmeester et al; licensee BioMed Central Ltd. 2010
Received: 25 September 2009
Accepted: 12 April 2010
Published: 12 April 2010
There is growing evidence that both local and systemic inflammatory responses play an important role in the progression of a variety of solid tumors. Colorectal cancer results from the cumulative effect of sequential genetic alterations, leading to the expression of tumor associated antigens possibly inducing a cellular anti-tumor immune response. It is well recognized that cytotoxic lymphocytes constitute one of the most important effector mechanisms of anti-tumor-immunity. However, their potential prognostic influence in colorectal cancer remains controversial. Aim of the study was to examine infiltration of CD3+ and CD8+ lymphocytes in colorectal cancer and their prognostic potential.
Two-hundred-fifteen colorectal cancer cases, previously analyzed for microsatellite instability (MSI), were selected for immunohistochemical detection of CD3+, CD8+ infiltration and the expression of granzyme B. Prognostic relevance was assessed by survival analysis.
Strong correlations were found between the infiltration of lymphocytes and several clinicopathological variables. Survival analysis revealed that intra-epithelial infiltration of CD3+ and CD8+ T lymphocytes and stromal infiltration of CD3+ lymphocytes had a major impact on the patients' overall survival in the univariate analysis, however independent of their association with MSI-status. In addition, it was also demonstrated that there was an important disease specific survival advantage for patients with microsatellite stable (MSS) tumors containing intraepithelial CD8+ tumor infiltrating lymphocytes. When samples were analyzed for colon cancer and rectal cancer separately, the results of the overall population were confirmed in colon cancer only. When entered into a multiple Cox regression analysis adjusting for other possible important confounding factors, the strong impact of lymphocyte infiltration on overall survival was not maintained. Only early stage and young age (borderline significant for overall population only) were associated with a better overall survival (early disease with disease-free survival also).
In conclusion our results suggest a role for infiltrating CD3+ and CD8+ T lymphocytes in colorectal cancer whereby tumor infiltration could reflect a general principle of antitumor immunity, irrespective of the MSI-status.
Colorectal cancer belongs to the most common malignancies in the Western World . The treatment of choice remains surgical resection. For patients who undergo successful surgery for colorectal cancer, adjuvant chemotherapy and/or radiotherapy is recommended in cases of high risk stage II and III disease [2, 3]. Although the introduction of new chemotherapeutic agents improved the prognosis of colorectal cancer over the past decades, the outlook for most patients still remains relative poor [1, 3]. Therefore, new treatment options, besides the standard therapies, seem warranted to further improve survival of patients with colorectal cancer , especially for stage II disease [5, 6]. New approaches focus on immunotherapeutic strategies as there is growing evidence in recent years supporting the existence of cancer immunosurveillance .
It has been recognized that disease progression in cancer patients is not solely determined by the characteristics of the tumor but also by the host response. Indeed, there is growing evidence that both local and systemic inflammatory responses play an important role in the progression of a variety of solid tumors [7–10]. In addition, the interrelationship between both inflammatory responses might have an influence on the outcome of the disease [7–10]. Colorectal carcinogenesis is a multistep process, during which (epi)genetic alterations determine the transition from a normal to a malignant cell. Acquisition of these alterations requires, among others, destabilization of the genome. Several forms of genetic instability (microsatellite instability (MSI), chromosomal instability and epigenetic instability) are believed to be involved in the development of colorectal cancer. MSI can lead to the production of abnormal proteins and derived peptides that, by acting as neo-antigens , could induce an adaptive immune response effective in limiting tumor growth and/or spread [11–16]. Nevertheless, the antitumor immune response is complex, involving the interaction of several cell types and cell products of the adaptive as well as the innate immune system [7, 17]. On the other hand, colorectal tumors are also capable of escaping immune surveillance using several strategies .
It is well recognized that cytotoxic T lymphocytes (CD8+ T cells) constitute one of the most important effector mechanisms of anti-tumor immunity . In order for CD8+ T cells to recognize antigens, these need to be exposed on the tumor cells in association with the human leukocyte antigen (HLA) class I proteins. Upon encounter of a tumor cell antigen/HLA I complex for which their T cell receptor is specific, cytotoxic T lymphocytes clonally expand and subsequently differentiate. Part of the differentiation process into killer cells includes the formation of a large number of modified lysosomes loaded with perforin and several types of granzymes [19, 20]. These activated cytotoxic T lymphocytes can mediate specific destruction of tumor cells by the release of these lytic components in case of direct cell-cell interaction. Perforin and enzymatic proteases (such as granzyme B) are released and cause cell death by disruption of the cell membrane and activation of the apoptotic pathway respectively [17, 21]. In addition, other factors of the adaptive immune system play a role in the cancer immunosurveillance. CD4+ T cells, which only respond to antigens presented by the HLA class II proteins expressed by antigen presenting cells (like dendritic cells), are important for antitumor immunity. Depending on the cytokine profile induced, CD4+ T cells are subdivided in T helper-1 cells or T helper-2 cells. Importantly, T helper-1 cells are essential for the proliferation of cytotoxic T lymphocytes as these lymphocytes require T helper-1 effector cell produced interleukin-2 for their proliferation. Although it has been shown that CD4+ T cells may be sufficient by themselves to eliminate tumor cells, it is more often the case that both CD4+ and CD8+ T cells are required for effective tumor cell elimination because most tumor cells only express HLA class I molecules. However, the induction of cytotoxic T lymphocytes responses takes time, leaving time for the tumor cells to escape the immune system. Therefore, natural killer cells from the innate immune system may also play an important role since these cells can lyse natural killer-sensitive tumor targets prior to antigen sensitization or clonal expansion. Given that natural killer cells are not HLA restricted, these cells bear the capacity to eliminate tumor cells that do not express HLA. In contrast, HLA complexes are recognized by inhibitory receptors on natural killer cells resulting in their inactivation. In addition, natural killer cells express several ligands of the tumor necrosis factor family and can induce apoptosis of malignant cell targets which are phagocytosed by dendritic cells and macrophages and processed for subsequent presentation to T cells. Furthermore, natural killer cells constitutively express the interleukin-2 receptor and are able to respond to interleukin-2 stimulation that results in augmented cytotoxic activity .
The potential influence of immune-cell infiltrates in various neoplasms, including colorectal cancer, on the prognosis of patients is investigated in several studies but remains inconclusive [4, 22, 23]. Pronounced lymphocyte infiltration in colorectal cancer has been described and is more marked in MSI-positive tumors [15, 16, 24–27]. Additionally, these tumors predominantly have a proximal location and are reported to run a better clinical course (as reviewed in [28, 29]). Some groups found that stage III colorectal tumors with a high content of intratumoral lymphocytes had a more favorable clinical outcome [30, 31]. In contrast, in a randomized trial it was demonstrated that active specific immunotherapy, using autologous tumor cells and Bacillus Calmette-Guérin, had a significant clinical benefit in the adjuvant treatment of stage II colon cancer only [32, 33].
Since several studies [13, 30, 34] showed the presence of a cytotoxic antitumor immune response in subsets of colorectal cancer, the aim of this study was to examine infiltration of CD3+ and CD8+ lymphocytes in colorectal cancer according to the classification of Naito et al. . Correlation with overall and disease-free survival was calculated in order to determine the potential prognostic role of cytotoxic T lymphocytes.
Total no. of patients
Median age (years)
70 +/- 12
64 +/- 13
68 +/- 13
Grade of differentiation
To enhance the power of the study the prognostic relevance of lymphocyte infiltration was assessed based on the comparison of two levels of infiltration: nil and mild infiltration on the one hand and moderate and severe infiltration on the other hand.
Semiquantitative scoring of inflammatory infiltration in colorectal cancer.
Strong associations were found between all infiltrating lymphocytes in the different tumor regions, except for CD3+ T cells and granzyme B expression in the invasive margin and the stroma. In addition, several strong correlations were found between the infiltration of lymphocytes and clinicopathological variables, such as poorly differentiated tumors (CD8+ in stroma and cancer cells, CD3+ and granzyme B in invasive margin), distal location (CD3+ in cancer cells and granzyme B in invasive margin), early stage (CD8+ in cancer cells, CD3+ in invasive margin and cancer cells and granzyme B in cancer cells) and the absence of treatment (CD8+ in all areas CD3+ and granzyme B in invasive margin and stroma).
Correlation of MSI and lymphocyte infiltration
As previously mentioned, all samples had been typed for MSI using the mononucleotide multiplex system . Twenty-seven out of 215 colorectal samples (12.6%) showed a high level of MSI (MSI-H) and 188 out of 215 showed microsatellite stability (MSS).
In this study population, sporadic MSI-H tumors were associated with proximal location (p < 0.001), poor differentiation (p < 0.001), female gender (p = 0.018) and there was a trend towards early stage (p = 0.077). A higher infiltration of CD8+ and CD3+ lymphocytes was noted in all examined areas of MSI-H tumors compared to MSS tumors. Granzyme B expression was considerably higher within the cancer cell nests of MSI-H tumors. In addition, logistic regression showed that MSI (HR: 5.505, p < 0.001) and stage (HR: 0.582, p = 0.006) were the major predictors of the presence of activated intraepithelial CD8+ T lymphocytes whereas grading, age, location and gender did not show a major impact.
Correlations with survival
Follow up for overall survival was available for 209 colorectal cancer patients. At the end of the observation period, 100 patients (47.8%) were deceased and all deaths were cancer related. Follow up for disease-free survival was available for 193 colorectal cancer patients and 57 patients (29.5%) experienced a recurrence of the tumor. The median follow up for overall and disease-free survival was 5.2 and 4.9 years respectively.
Survival analysis using a Cox regression model.
Disease free survival
Grade of differentiation
Discussion and Conclusions
Solid tumors are not just composed of malignant cells, but are complex microcosms of many cell types, including a wide range of hematopoietic cells . Anti-tumor immune responses may be one of the most important weapons in the arsenal against cancer . Galon et al. [38, 39] suggested that once human colorectal tumors become clinically detectable, the adaptive immune response plays a role in preventing tumor recurrence and metastasis. Intratumoral T cells could modify tumor stroma or tumor cells in ways that attenuate the metastatic potential of tumor cells [40, 41]. However, it is still unclear if the presence of tumor infiltrating lymphocytes represents the result of an inflammatory response that facilitates either tumor progression or a protective host response against cancer .
The present study confirms that tumor infiltrating lymphocytes are present in colorectal cancer and, as expected, a strong correlation was found between CD3+ and CD8+ T cells. Additionally, a strong association was also seen between the presence of CD8+ T lymphocytes and the expression of their associated cytotoxic molecule granzyme B in all examined areas of the tumor. Granzyme B expression was much sparser which can be explained by the fact that only those CD8+ T lymphocytes which are cytolytic (about 50% in this study) will express their associated cytotoxic molecule granzyme B. When exposed to dysfunctional cells, cytotoxic T lymphocytes will release granzyme B and will eventually induce apoptosis of the proximal tumor cells bearing the appropriate antigen determinants [7, 17]. This might hold promise in the field of immunotherapy [16, 42]. Unfortunately, at this time we were unable to investigate T cell surface activation markers or the apoptosis status of the infiltrated tumor cells in order to confirm the lytic function of these infiltrating T cells.
As found in several other studies [7, 39, 43–48], the presence of a pronounced lymphocytic infiltration within the tumor is associated with improved survival in the current study. Specifically, CD3+ and CD8+ T lymphocytes within cancer cell nests of colorectal cancer and of CD3+ in the colorectal cancer stroma had a major impact on the patients' overall survival by univariate analysis in the overall population. When colon and rectal tumors were investigated separately, the results of the overall population were confirmed in the group of colon cancer patients but not in the group of the rectal cancer patients, even when the rectal cancer patients receiving pre-operative radiotherapy were excluded from the analysis.
The improved survival associated with infiltration of lymphocytes may be the result of an effective suppression of micrometastases in distant organs or near the primary site. In other words, the number of CD8+ T cells within the primary tumor might be a good indicator of the presence of a systemic immunosurveillance mechanism [40, 41]. In addition, tumor cells can secrete substances in the stroma, which might be recognized by the immune system to destroy the tumor.
In accordance to Pages et al. , Ropponen et al  and Koch et al , but in contrast to Ling et al. , stage was a major determinant of infiltration of activated cytotoxic intraepithelial lymphocytes (as shown by multivariate analysis) with a higher infiltration in earlier stages (HR: 0.582, p = 0.006). This might indicate that immune reactions may be more prominent in early stages of disease and might have a stage specific influence on survival [31, 50], possibly irrespective of the MSI-status. There might be a protective local immune response in these earlier tumor stages, preventing further tumor growth and spread. However, this hypothesis has to be substantiated by further analysis of activation status of tumor infiltrating lymphocytes in patients with early tumor stages. Alternatively, host immune response against cancer cells may decrease with increasing tumor growth .
Several groups found stage specific beneficial effects of infiltrating lymphocytes on clinical outcome [30–33]. Guidoboni et al. described a correlation between the presence of cytotoxic T lymphocytes and improved survival in stage II and III colorectal tumors irrespective of the MSI-status . These results were confirmed by Prall et al.  for stage III disease. However, when MSI-status was taken into account, the correlation with survival was most pronounced when both MSI-H and intratumoral-activated cytotoxic T lymphocytes were present in stage III tumors .
In this study, stage specific survival analysis did not show a major influence of tumor infiltration on overall survival. However, given the relative small number of cases per stage group, such an influence might be difficult to detect. Nevertheless, this could be of interest since we previously showed a significant beneficial effect of active specific immunotherapy in adjuvant treatment of patients with stage II colon cancer only .
Accumulating evidence indicates that most MSI-positive colorectal tumors are characterized by the presence of a pronounced intratumoral inflammatory reaction, the nature of which, however, is still poorly understood [30, 51]. Within these tumors, tumor infiltrating lymphocytes have been identified as predominantly activated CD8+ T cells. The presence of these cytotoxic T lymphocytes has been attributed to the inherently greater production of abnormal peptides as a result of unreliable DNA repair in MSI-positive tumors . In the current study, among the different clinicopathological parameters analyzed, MSI was strongly correlated with a high presence of activated cytotoxic intraepithelial lymphocytes. Nevertheless, survival analysis by MSI-status revealed that, despite the high infiltration of lymphocytes in MSI-positive colorectal tumors, no association was found with a favorable prognosis within this subset. These data are in agreement with some studies [31, 37, 50, 52–57], but they are in conflict with a number of other reports [13, 24–27, 30, 34, 58] in which a predominant presence of intra-tumor cell infiltrating lymphocytes in MSI-H cancers was significantly correlated with a better prognosis. In accordance to Baker et al. , MSS colorectal tumors with infiltration of CD8+ tumor infiltrating lymphocytes showed a better overall survival. One explanation might be that the functions of tumor infiltrating lymphocytes differ between these subgroups. It is possible that the high prevalence of recruitment and retention of tumor infiltrating lymphocytes in MSI-positive colorectal tumors is inherent to the unique aspect of the tumor biology, supporting the hypothesis that the genetic instability of MSI-positive colorectal tumors may lead to the production of a greater number of tumor specific antigens that trigger the immune system [7, 11–16, 27, 30]. As described previously , direct contact between tumor cells, presenting these aberrant peptides, and leucocytes plays a crucial role in the immune reaction. However, the concept of cross-priming in which antigen presenting cells pick up antigens released by dead tumor cells and subsequently present them to T cells may also be important in vivo .
On the other hand, MSI-positive tumors can undergo immuno-editing, which dictates that tumors reaching the stage of clinical detection have been shaped antigenically by the initial immune responses mounted against them to a point where they are no longer recognized as foreign to the body. In MSS tumors, which arise in a much less immunologically stimulating environment, the tumors are less likely to have adapted antigenically and may thus be more sensitive to late stage immune attack. Thus, even though MSI-positive tumors produce more tumor specific antigens that might trigger the immune system, the elevated levels of these antigens in combination with the lack of appropriate costimulatory molecules on the tumor cells may generate a microenvironment which leads to a state of tumor infiltrating lymphocyte anergy, thereby preempting any beneficial effect on survival [12, 37]. In addition, the expression of HLA class I proteins, presenting tumor associated antigens on the tumor cell surface, is considered a prerequisite for an effective T cell immune response . It has been described that genetically unstable tumors are often HLA class I negative and might escape T-cell mediated immune killing. However, in the absence of any surface HLA class I these tumors would be susceptible to a natural killer cell attack. In contrast, those tumors that downregulate specific HLA class I alleles may avoid both T cell and natural killer cell activation . Therefore, Speetjens et al.  hypothesized that both oncogenic pathway and HLA class I expression might dictate clinical tumor progression. Furthermore, insufficient evidence exists to recommend routine use of biological factors as MSI for either determining prognosis or predicting response to therapy in colorectal cancer patients .
When entered into a multiple Cox regression analysis adjusting for other possible important confounding factors, only early stage and young age (borderline significant for overall population only) were associated with a better overall survival (early stage also with disease-free survival). The beneficial effect of tumor infiltrating lymphocytes in colorectal cancer could not be confirmed in the Cox regression survival analysis.
In summary, this study confirms that tumor infiltrating lymphocytes are indeed important clinical and prognostic indicators in colorectal cancer, in univariate analysis, irrespective of the MSI-status. Therefore, it was considered that tumor infiltration could reflect a general principle of antitumor immunity as mentioned by Prall et al. . In addition, in accordance with Baker et al., we also demonstrated an important disease-specific survival advantage for patients with MSS tumors containing intraepithelial CD8+ tumor infiltrating lymphocytes . Although no stage specific survival difference of tumor infiltrating lymphocytes was noted in colorectal cancer, it was demonstrated that stage was also a major determinant of the presence of tumor infiltrating lymphocytes in colorectal cancer, with a higher infiltration in earlier stages.
We would like to note that the tumor immune response does not only depend on infiltration and activation of T lymphocytes. Therefore, the minute balanced association of all infiltrating cells, their capability to synthesize and release tumor-modifying substances, and their content of presynthesized, granule-associated bioactive substances deserves intensive study. By doing so, new biological tumor-host responses may be disclosed, leading to the development of new approaches to the treatment of malignant disease .
Tumor DNA was obtained from 215 patients with surgically resected colorectal cancer, treated between 1995 and 2003, from the Antwerp University Hospital and the St. Augustinus Hospital Antwerp. The study was approved by the local Ethics Committee of the University of Antwerp and was conducted in accordance with the ethical principles stated in the most recent version of the Declaration of Helsinki.
Five μm-thick sections were prepared from formalin-fixed paraffin-embedded tissue for IHC. Sections were deparaffinized in toluene, dehydrated and subjected to heat antigen retrieval by Tris EDTA buffer (pH 9) in a heating bath for 30 min. at 95 (± 1)°C.
Sections were subsequently stained using the Dako Autostainer Plus system (DAKO, DakoCytomation, Glostrup, Denmark). Endogenous peroxidase activity was quenched by incubating the slides in peroxidase block EnVision Plus (DAKO EnVision™ kit, DakoCytomation, Glostrup, Denmark) for 10 minutes. Incubation with primary monoclonal antibodies against: CD3 (clone SPF7, diluted 1:150, Neo-Markers, CA, USA), CD8 (clone 1A5, diluted 1:20, Novacastra Laboratories Ltd, Newcastle upon Tyne, UK) and Granzyme B (clone GRB7, diluted 1:25, DAKO, DakoCytomation, Glostrup, Denmark) was performed for 30 minutes at room temperature. Sites of binding were detected using the Envision dual link detection system (DAKO EnVision™ kit, DakoCytomation, Glostrup, Denmark) with 3,3'-diaminobenzidine (DAB+) as chromogen according to the manufacturer's instructions. The sections were counterstained with haematoxylin, dehydrated, cleared and mounted.
Classification and quantification of lymphocytes
Inflammatory infiltration by CD3+ (early T cell marker), CD8+(cytotoxic T cell marker) T lymphocytes and the expression of Granzyme B (marker for cytotoxic cells after cell activation) were immunohistochemically scored in three regions of the tumor as described by Naito et al. : a) those distributed along the invasive margin of the tumor, b) those infiltrated in cancer stroma and c) those infiltrated in the tumor cell nests.
Each entire tumor section was evaluated for infiltrating cells by using a × 10 objective lens. The number of lymphocytes infiltrating the tumor cell nests was counted in five visual fields, selected for most abundant distribution under a light microscope at × 400 magnification, corresponding to a total area of 1.19 mm2. The degree of infiltration was classified as follows: nil: 0, mild: 1-19, moderate: 20-49 and severe: ≥ 50 positive cells .
For all other regions of the tumor, immune reactivity was scored semi-quantitatively (nil, mild, moderate and severe) as described by Naito et al.,  Scoring was performed by two independent observers, if no consensus could be reached a third observer was consulted.
All cases had been previously analyzed for MSI-status . After manual microdissection of formalin-fixed, paraffin embedded tissue blocks, DNA was isolated as described previously . MSI analysis was performed using the mononucleotide multiplex system as described earlier . In short, the sense primers were chemically labeled at the 5' end with FAM™ fluorescent dyes. PCR was carried out in a final volume of 25 μl containing 200 μmol/L dNTPs (MBI Fermentas, St. Leon-Rot, Germany), 500 nM of each sense and antisense primer (Eurogentec, Seraing, Belgium), 1 × PCR buffer (60 mM Tris SO4 (pH 8.9), 18 mM (NH4)SO4 and 2 mM MgSO4) and 1 unit Discoverase dHPLC DNA polymerase (Invitrogen, Merelbeke, Belgium). Fluorescent PCR products were analyzed by capillary electrophoresis using an ABI 3100 Genetic Analyzer (Applied Biosystems, Lennik, Belgium) and Genemapper Software 3.7.
Prognostic relevance of lymphocyte infiltration was assessed by survival analysis.
The index date for survival time calculation was defined as the date of surgery for colorectal cancer. The months of observation (= overall survival time) were calculated from the index date to the date of last information/death. For disease free survival time the months of observation were calculated from the index date to the first date of progressive disease. Survival curves were determined by using the Kaplan-Meier method and were analyzed by using the log-rank test.
Possible associations between MSI-status and the immunophenotypic markers and clinicopathological parameters of colorectal cancers were investigated using the χ2-test or Fisher's exact test (when numbers expected per cell are less than 5) for categorical variables and using Student t-test (for unpaired comparisons based on numerical variables for which the assumption of a normal distribution was appropriate (i.e. Kolmogorov-Smirnoff test p > 0.02)) or its nonparametric alternative Mann-Whitney U test (for unpaired comparisons based on numerical variables for which the assumption of a normal distribution was not appropriate) for continuous variables. In order to assess the independent prognostic contribution of the cytotoxic T lymphocytes, a multiple Cox regression analysis was conducted. All analyses were conducted using SPSS (version 16.0). Significance for all statistics was recorded if p < 0.05 (two-tailed).
The authors gratefully acknowledge support of the Special Research Fund of the University of Antwerp, Belgium, and are indebted to Mr. Willy Floren (Schilde, Belgium) for his private donation. In addition, we would like to thank the laboratory technicians of the laboratory of Pathology for performing the immunohistochemical stainings. Last but not least we would like to express our gratitude to Dr. Trinh of the Translational Cancer Research Group for his help in sifting through the medical files.
- Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ: Cancer Statistics, 2009. CA Cancer J Clin. 2009, 59: 225-249. 10.3322/caac.20006.View ArticlePubMedGoogle Scholar
- Weitz J, Koch M, Debus J, Hohler T, Galle PR, Buchler MW: Colorectal cancer. Lancet. 2005, 365: 153-165. 10.1016/S0140-6736(05)17706-X.View ArticlePubMedGoogle Scholar
- Lurje G, Zhang W, Lenz HJ: Molecular prognostic markers in locally advanced colon cancer. Clin Colorectal Cancer. 2007, 6: 683-690. 10.3816/CCC.2007.n.037.View ArticlePubMedGoogle Scholar
- Koch M, Beckhove P, Op den Winkel J, Autenrieth D, Wagner P, Nummer D, et al.,: Tumor infiltrating T lymphocytes in colorectal cancer: Tumor-selective activation and cytotoxic activity in situ. Ann Surg. 2006, 244: 986-992. 10.1097/01.sla.0000247058.43243.7b.PubMed CentralView ArticlePubMedGoogle Scholar
- NIH consensus conference. Adjuvant therapy for patients with colon and rectal cancer. JAMA. 1990, 264: 1444-1450. 10.1001/jama.264.11.1444.View ArticleGoogle Scholar
- Shulman K, Schilsky R: Adjuvant therapy of colon cancer. Semin Oncol. 1995, 22: 600-10.PubMedGoogle Scholar
- Dalerba P, Maccalli C, Casati C, Castelli C, Parmiani G: Immunology and immunotherapy of colorectal cancer. Crit Rev Oncol Hematol. 2003, 46: 33-57. 10.1016/S1040-8428(02)00159-2.View ArticlePubMedGoogle Scholar
- Canna K, McArdle PA, McMillan DC, McNicol AM, Smith GW, McKee RF, et al, et al.,: The relationship between tumour T-lymphocyte infiltration, the systemic inflammatory response and survival in patients undergoing curative resection for colorectal cancer. Br J Cancer. 2005, 92: 651-654. 10.1038/sj.bjc.6602419.PubMed CentralView ArticlePubMedGoogle Scholar
- Roxburgh CS, Salmond JM, Horgan PG, Oien KA, McMillan DC: Comparison of the prognostic value of inflammation-based pathologic and biochemical criteria in patients undergoing potentially curative resection for colorectal cancer. Ann Surg. 2009, 249: 788-793. 10.1097/SLA.0b013e3181a3e738.View ArticlePubMedGoogle Scholar
- Roxburgh CS, Salmond JM, Horgan PG, Oien KA, McMillan DC: Tumour inflammatory infiltrate predicts survival following curative resection for node-negative colorectal cancer. Eur J Cancer. 2009, 45: 2138-2145. 10.1016/j.ejca.2009.04.011.View ArticlePubMedGoogle Scholar
- Linnebacher M, Gebert J, Rudy W, Woerner S, Yuan Y, Bork P, et al.,: Frameshift peptide-derived T-cell epitopes: a source of novel tumor-specific antigens. Int J Cancer. 2001, 93: 6-11. 10.1002/ijc.1298.View ArticlePubMedGoogle Scholar
- Diederichsen AC, Hjelmborg JB, Christensen PB, Zeuthen J, Fenger C: Prognostic value of the CD4+/CD8+ ratio of tumour infiltrating lymphocytes in colorectal cancer and HLA-DR expression on tumour cells. Cancer Immunol Immunother. 2003, 52: 423-428. 10.1007/s00262-003-0388-5.View ArticlePubMedGoogle Scholar
- Guidoboni M, Gafa R, Viel A, Doglioni C, Russo A, Santini A, et al.,: Microsatellite instability and high content of activated cytotoxic lymphocytes identify colon cancer patients with a favorable prognosis. Am J Pathol. 2001, 159: 297-304.PubMed CentralView ArticlePubMedGoogle Scholar
- Ishikawa T, Fujita T, Suzuki Y, Okabe S, Yuasa Y, Iwai T, et al.,: Tumor-specific immunological recognition of frameshift-mutated peptides in colon cancer with microsatellite instability. Cancer Res. 2003, 63: 5564-5572.PubMedGoogle Scholar
- Drescher KM, Sharma P, Watson P, Gatalica Z, Thibodeau SN, Lynch HT: Lymphocyte recruitment into the tumor site is altered in patients with MSI-H colon cancer. Fam Cancer. 2009, 8: 231-239. 10.1007/s10689-009-9233-0.View ArticlePubMedGoogle Scholar
- Schwitalle Y, Kloor M, Eiermann S, Linnebacher M, Kienle P, Knaebel HP, et al.,: Immune response against frameshift-induced neopeptides in HNPCC patients and healthy HNPCC mutation carriers. Gastroenterology. 2008, 134: 988-997. 10.1053/j.gastro.2008.01.015.View ArticlePubMedGoogle Scholar
- Titu LV, Monson JR, Greenman J: The role of CD8(+) T cells in immune responses to colorectal cancer. Cancer Immunol Immunother. 2002, 51: 235-247. 10.1007/s00262-002-0276-4.View ArticlePubMedGoogle Scholar
- Evans C, Dalgleish AG, Kumar D: Review article: immune suppression and colorectal cancer. Aliment Pharmacol Ther. 2006, 24: 1163-1177. 10.1111/j.1365-2036.2006.03075.x.View ArticlePubMedGoogle Scholar
- Loose D, Wiele Van de C: The immune system and cancer. Cancer Biother Radiopharm. 2009, 24: 369-376. 10.1089/cbr.2008.0593.View ArticlePubMedGoogle Scholar
- Watson NF, Ramage JM, Madjd Z, Spendlove I, Ellis IO, Scholefield JH, et al.,: Immunosurveillance is active in colorectal cancer as downregulation but not complete loss of MHC class I expression correlates with a poor prognosis. Int J Cancer. 2006, 118: 6-10. 10.1002/ijc.21303.View ArticlePubMedGoogle Scholar
- Atkinson EA, Bleackley RC: Mechanisms of lysis by cytotoxic T cells. Crit Rev Immunol. 1995, 15: 359-384.View ArticlePubMedGoogle Scholar
- Parmiani G: Tumor-infiltrating T cells--friend or foe of neoplastic cells?. N Engl J Med. 2005, 353: 2640-1. 10.1056/NEJMp058236.View ArticlePubMedGoogle Scholar
- Klintrup K, Makinen JM, Kauppila S, Vare PO, Melkko J, Tuominen H, et al.,: Inflammation and prognosis in colorectal cancer. Eur J Cancer. 2005, 41: 2645-2654. 10.1016/j.ejca.2005.07.017.View ArticlePubMedGoogle Scholar
- Naito Y, Saito K, Shiiba K, Ohuchi A, Saigenji K, Nagura H, et al.,: CD8+ T cells infiltrated within cancer cell nests as a prognostic factor in human colorectal cancer. Cancer Res. 1998, 58: 3491-4.PubMedGoogle Scholar
- Smyrk TC, Watson P, Kaul K, Lynch HT: Tumor-infiltrating lymphocytes are a marker for microsatellite instability in colorectal carcinoma. Cancer. 2001, 91: 2417-2422. 10.1002/1097-0142(20010615)91:12<2417::AID-CNCR1276>3.0.CO;2-U.View ArticlePubMedGoogle Scholar
- Takemoto N, Konishi F, Yamashita K, Kojima M, Furukawa T, Miyakura Y, et al.,: The correlation of microsatellite instability and tumor-infiltrating lymphocytes in hereditary non-polyposis colorectal cancer (HNPCC) and sporadic colorectal cancers: the significance of different types of lymphocyte infiltration. Jpn J Clin Oncol. 2004, 34: 90-98. 10.1093/jjco/hyh018.View ArticlePubMedGoogle Scholar
- Phillips SM, Banerjea A, Feakins R, Li SR, Bustin SA, Dorudi S: Tumour-infiltrating lymphocytes in colorectal cancer with microsatellite instability are activated and cytotoxic. Br J Surg. 2004, 91: 469-475. 10.1002/bjs.4472.View ArticlePubMedGoogle Scholar
- Popat S, Hubner R, Houlston R: Systematic review of microsatellite instability and colorectal cancer prognosis. J Clin Oncol. 2005, 23: 609-18. 10.1200/JCO.2005.01.086.View ArticlePubMedGoogle Scholar
- Chang S, Lin J, Yang S, Wang H, Li A, Chi C: Relationship between genetic alterations and prognosis in sporadic colorectal cancer. Int J Cancer. 2006, 118: 1721-7. 10.1002/ijc.21563.View ArticlePubMedGoogle Scholar
- Dolcetti R, Viel A, Doglioni C, Russo A, Guidoboni M, Capozzi E, et al.,: High prevalence of activated intraepithelial cytotoxic T lymphocytes and increased neoplastic cell apoptosis in colorectal carcinomas with microsatellite instability. Am J Pathol. 1999, 154: 1805-13.PubMed CentralView ArticlePubMedGoogle Scholar
- Prall F, Duhrkop T, Weirich V, Ostwald C, Lenz P, Nizze H, et al.,: Prognostic role of CD8+tumor-infiltrating lymphocytes in stage III colorectal cancer with and without microsatellite instability. Human Path. 2004, 35: 808-816. 10.1016/j.humpath.2004.01.022.View ArticleGoogle Scholar
- Vermorken JB, Claessen AM, van Tinteren H, Gall HE, Ezinga R, Meijer S, et al.,: Active specific immunotherapy for stage II and stage III human colon cancer: a randomised trial. Lancet. 1999, 353: 345-50. 10.1016/S0140-6736(98)07186-4.View ArticlePubMedGoogle Scholar
- Eertwegh van den AJ: Active specific immunotherapy in colon cancer. Recent Results Cancer Res. 2005, 165: 260-267. full_text.View ArticlePubMedGoogle Scholar
- Michael-Robinson JM, Biemer-Huttmann AE, Purdie DM, Walsh MD, Simms LA, Biden KG, et al.,: Tumour infiltrating lymphocytes and apoptosis are independent features in colorectal cancer stratified according to microsatellite instability status. Gut. 2001, 48: 360-366. 10.1136/gut.48.3.360.PubMed CentralView ArticlePubMedGoogle Scholar
- Deschoolmeester V, Baay M, Wuyts W, Van ME, Van DN, Vermeulen P, et al.,: Detection of microsatellite instability in colorectal cancer using an alternative multiplex assay of quasi-monomorphic mononucleotide markers. J Mol Diagn. 2008, 10: 154-159. 10.2353/jmoldx.2008.070087.PubMed CentralView ArticlePubMedGoogle Scholar
- Lin EY, Pollard JW: Role of infiltrated leucocytes in tumour growth and spread. Br J Cancer. 2004, 90: 2053-2058. 10.1038/sj.bjc.6601705.PubMed CentralView ArticlePubMedGoogle Scholar
- Baker K, Zlobec I, Tornillo L, Terracciano L, Jass JR, Lugli A: Differential significance of tumour infiltrating lymphocytes in sporadic mismatch repair deficient versus proficient colorectal cancers: a potential role for dysregulation of the transforming growth factor-beta pathway. Eur J Cancer. 2007, 43: 624-631. 10.1016/j.ejca.2006.11.012.View ArticlePubMedGoogle Scholar
- Galon J, Costes A, Sanchez-Cabo F, Kirilovsky A, Mlecnik B, Lagorce-Pages C, et al.,: Type, density, and location of immune cells within human colorectal tumors predict clinical outcome. Science. 2006, 313: 1960-1964. 10.1126/science.1129139.View ArticlePubMedGoogle Scholar
- Galon J, Fridman WH, Pages F: The adaptive immunologic microenvironment in colorectal cancer: a novel perspective. Cancer Res. 2007, 67: 1883-1886. 10.1158/0008-5472.CAN-06-4806.View ArticlePubMedGoogle Scholar
- Camus M, Tosolini M, Mlecnik B, Pages F, Kirilovsky A, Berger A, et al.,: Coordination of intratumoral immune reaction and human colorectal cancer recurrence. Cancer Res. 2009, 69: 2685-2693. 10.1158/0008-5472.CAN-08-2654.View ArticlePubMedGoogle Scholar
- Chiba T, Ohtani H, Mizoi T, Naito Y, Sato E, Nagura H, et al.,: Intraepithelial CD8+ T-cell-count becomes a prognostic factor after a longer follow-up period in human colorectal carcinoma: possible association with suppression of micrometastasis. Br J Cancer. 2004, 91: 1711-1717.PubMed CentralPubMedGoogle Scholar
- Banerjea A, Bustin S, Dorudi S: The immunogenicity of colorectal cancers with high-degree microsatellite instability. World J Surg Oncol. 2005, 3: 26-10.1186/1477-7819-3-26.PubMed CentralView ArticlePubMedGoogle Scholar
- Jass JR, Love SB, Northover JM: A new prognostic classification of rectal cancer. Lancet. 1987, 1: 1303-1306. 10.1016/S0140-6736(87)90552-6.View ArticlePubMedGoogle Scholar
- Ropponen KM, Eskelinen MJ, Lipponen PK, Alhava E, Kosma VM: Prognostic value of tumour-infiltrating lymphocytes (TILs) in colorectal cancer. J Pathol. 1997, 182: 318-324. 10.1002/(SICI)1096-9896(199707)182:3<318::AID-PATH862>3.0.CO;2-6.View ArticlePubMedGoogle Scholar
- Nielsen HJ, Hansen U, Christensen IJ, Reimert CM, Brunner N, Moesgaard F: Independent prognostic value of eosinophil and mast cell infiltration in colorectal cancer tissue. J Pathol. 1999, 189: 487-495. 10.1002/(SICI)1096-9896(199912)189:4<487::AID-PATH484>3.0.CO;2-I.View ArticlePubMedGoogle Scholar
- Funada Y, Noguchi T, Kikuchi R, Takeno S, Uchida Y, Gabbert HE: Prognostic significance of CD8+ T cell and macrophage peritumoral infiltration in colorectal cancer. Oncol Rep. 2003, 10: 309-313.PubMedGoogle Scholar
- Pages F, Berger A, Camus M, Sanchez Cabo F, Costes A, Molidor R, et al.,: Effector memory T cells, early metastasis, and survival in colorectal cancer. N Engl J Med. 2005, 353: 2654-66. 10.1056/NEJMoa051424.View ArticlePubMedGoogle Scholar
- Halama N, Michel S, Kloor M, Zoernig I, Pommerencke T, von Knebel DM, et al.,: The localization and density of immune cells in primary tumors of human metastatic colorectal cancer shows an association with response to chemotherapy. Cancer Immun. 2009, 9: 1-PubMed CentralPubMedGoogle Scholar
- Ling KL, Pratap SE, Bates GJ, Singh B, Mortensen NJ, George BD, et al.,: Increased frequency of regulatory T cells in peripheral blood and tumour infiltrating lymphocytes in colorectal cancer patients. Cancer Immun. 2007, 7: 7-PubMed CentralPubMedGoogle Scholar
- Salahshor S, Kressner U, Fischer H, Lindmark G, Glimelius B, Pahlman L, et al.,: Microsatellite instability in sporadic colorectal cancer is not an independent prognostic factor. Br J Cancer. 1999, 81: 190-3. 10.1038/sj.bjc.6690676.PubMed CentralView ArticlePubMedGoogle Scholar
- Fujiwara T, Stolker J, Watanabe T, Rashid A, Longo P, Eshleman J, et al.,: Accumulated clonal genetic alterations in familial and sporadic colorectal carcinomas with widespread instability in microsatellite sequences. Am J Pathol. 1998, 153: 1063-78.PubMed CentralView ArticlePubMedGoogle Scholar
- Feeley KM, Fullard JF, Heneghan MA, Smith T, Maher M, Murphy RP, et al.,: Microsatellite instability in sporadic colorectal carcinoma is not an indicator of prognosis. J Pathol. 1999, 188: 14-17. 10.1002/(SICI)1096-9896(199905)188:1<14::AID-PATH323>3.0.CO;2-Q.View ArticlePubMedGoogle Scholar
- Messerini L, Ciantelli M, Baglioni S, Palomba A, Zampi G, Papi L: Prognostic significance of microsatellite instability in sporadic mucinous colorectal cancers. Hum Pathol. 1999, 30: 629-34. 10.1016/S0046-8177(99)90086-2.View ArticlePubMedGoogle Scholar
- Curran B, Lenehan K, Mulcahy H, Tighe O, Bennett MA, Kay EW, et al.,: Replication error phenotype, clinicopathological variables, and patient outcome in Dukes' B stage II (T3, N0, M0) colorectal cancer. Gut. 2000, 46: 200-4. 10.1136/gut.46.2.200.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang C, van Rijnsoever M, Grieu F, Bydder S, Elsaleh H, Joseph D, et al.,: Prognostic significance of microsatellite instability and Ki-ras mutation type in stage II colorectal cancer. Oncology. 2003, 64: 259-65. 10.1159/000069311.View ArticlePubMedGoogle Scholar
- Emterling A, Wallin A, Arbman G, Sun XF: Clinicopathological significance of microsatellite instability and mutated RIZ in colorectal cancer. Ann Oncol. 2004, 15: 242-246. 10.1093/annonc/mdh045.View ArticlePubMedGoogle Scholar
- Storojeva I, Boulay J, Heinimann K, Ballabeni P, Terracciano L, Laffer U, et al.,: Prognostic and predictive relevance of microsatellite instability in colorectal cancer. Oncol Rep. 2005, 14: 241-9.PubMedGoogle Scholar
- Buckowitz A, Knaebel H, Benner A, Blaker H, Gebert J, Kienle P, et al.,: Microsatellite instability in colorectal cancer is associated with local lymphocyte infiltration and low frequency of distant metastases. Br J Cancer. 2005, 92: 1746-53. 10.1038/sj.bjc.6602534.PubMed CentralView ArticlePubMedGoogle Scholar
- Menon AG, Janssen-van Rhijn CM, Morreau H, Putter H, Tollenaar RA, Velde van de CJH, et al.,: Immune system and prognosis in colorectal cancer: a detailed immunohistochemical analysis. Lab Invest. 2004, 84: 493-501. 10.1038/labinvest.3700055.View ArticlePubMedGoogle Scholar
- Speetjens FM, de Bruin EC, Morreau H, Zeestraten EC, Putter H, van Krieken JH, et al.,: Clinical impact of HLA class I expression in rectal cancer. Cancer Immunol Immunother. 2008, 57: 601-609. 10.1007/s00262-007-0396-y.PubMed CentralView ArticlePubMedGoogle Scholar
- Duffy MJ, van DA, Haglund C, Hansson L, Holinski-Feder E, Klapdor R, et al.,: Tumour markers in colorectal cancer: European Group on Tumour Markers (EGTM) guidelines for clinical use. Eur J Cancer. 2007, 43: 1348-1360. 10.1016/j.ejca.2007.03.021.View ArticlePubMedGoogle Scholar
- Deschoolmeester V, Van Damme N, Baay M, Claes K, Van ME, Baert FJ, et al.,: Microsatellite instability in sporadic colon carcinomas has no independent prognostic value in a Belgian study population. Eur J Cancer. 2008, 44: 2288-2295. 10.1016/j.ejca.2008.06.043.View ArticlePubMedGoogle Scholar
- Deschoolmeester V, Baay M, Wuyts W, Van ME, Pelckmans P, Lardon F, et al.,: Comparison of three commonly used PCR-based techniques to analyze MSI status in sporadic colorectal cancer. J Clin Lab Anal. 2006, 20: 52-61. 10.1002/jcla.20097.View ArticlePubMedGoogle Scholar
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