Figure 3

Adoptive transfer of B/I activated T cells into CYP pretreated hosts results in tumor specific production of interferon-γ and other cytotoxic mediators by adoptive host splenocytes. A) RT-PCR analysis of granzyme B, perforin, FasL and actin mRNA from murine 4T1 tumor-DLN CD8⁺ cells, either fresh or after activation with B/I and expansion in IL-2. Increased expression of FasL, perforin, and granzyme B was seen in CD8+ T cells after B/I activation. This is a representative experiment of 3 replicates. B) ELIspot assays for IFN-γ producing cells from spleens of untreated 4T1 tumor-bearing mice, CYP-treated tumor-bearing mice, tumor bearing mice treated with CYP + AIT with B/I-activated tDLN, and 4T1 bearing mice immunized with irradiated 4T1 cells. Splenocytes were unstimulated or stimulated in vitro with 4T1 or Meth A sarcoma cells. Results are shown for pooled spleens from 3 mice in each group. Mean values from paired wells are shown. This is a representative experiment of 5 replicates.