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Table 3 Absolute numbers of adoptively transferred cells in lymph nodes of tumor-bearing mice

From: Phenotype, functions and fate of adoptively transferred tumor draining lymphocytes activated ex vivo in mice with an aggressive weakly immunogenic mammary carcinoma

 

Day 3

Day 5

Day 7

Day 10

tDLN

4.6 × 105

3.9 × 105

2.8 × 105

2.1 × 105

cLN

9.1 × 104

2.3 × 103

1.8 × 104

6.4 × 104

  1. Mice with established 4T1 flank tumors were either untreated, treated with CYP alone on day 9, or were treated with CYP on Day 9 after tumor inoculation followed by AIT with 50 × 106 CFSE-stained B/I activated 4T1 tDLN (on day 10). At 1 hour, 3, 5, 7, and 10 days after AIT, the tDLN and contralateral LN (cLN) were harvested and analyzed by flow cytometry for CFSE+ cells. This is a representative experiment of 5 replicates. Absolute numbers of cells found in each tissue are listed.