| Day 3 | Day 5 | Day 7 | Day 10 |
---|
tDLN
| 4.6 × 105 | 3.9 × 105 | 2.8 × 105 | 2.1 × 105 |
cLN
| 9.1 × 104 | 2.3 × 103 | 1.8 × 104 | 6.4 × 104 |
- Mice with established 4T1 flank tumors were either untreated, treated with CYP alone on day 9, or were treated with CYP on Day 9 after tumor inoculation followed by AIT with 50 × 106 CFSE-stained B/I activated 4T1 tDLN (on day 10). At 1 hour, 3, 5, 7, and 10 days after AIT, the tDLN and contralateral LN (cLN) were harvested and analyzed by flow cytometry for CFSE+ cells. This is a representative experiment of 5 replicates. Absolute numbers of cells found in each tissue are listed.