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Table 4 CD8+ T cells infiltrate 4T1 tumors after AIT treatment

From: Phenotype, functions and fate of adoptively transferred tumor draining lymphocytes activated ex vivo in mice with an aggressive weakly immunogenic mammary carcinoma

Group

Nil

CD4

CD8a

Day 1: Control

-

+

-

Cyp

-

_

-

AIT/CYP

-

++

++

Day 4: Control

-

+

-

CYP

-

++

-

AIT/CYP

-

+++

+

Day 7: Control

-

+

-

CYP

-

+

-

AIT/CYP

-

+

+

Day 11: Control

-

++

-

CYP

-

+

-

AIT/CYP

-

+++

+

  1. Grading:
  2. - No stain
  3. + 1-15%
  4. ++ 16-50%
  5. +++ >50%
  6. Mice with established 4T1 flank tumors were either untreated, treated with CYP alone on day 3, or were treated with CYP on Day 3 after tumor inoculation followed by AIT with 25 × 106 B/I activated 4T1 tDLN (on day 4). At 1, 4, 7 and 11 days after AIT, the tumors were harvested and tumor cross-sections were stained for CD4 and CD8 expression. A representative experiment is shown.