Group
| Nil | CD4 | CD8a |
---|
Day 1: Control | - |
+
| - |
Cyp | - |
_
| - |
AIT/CYP | - |
++
|
++
|
Day 4: Control | - |
+
| - |
CYP | - |
++
| - |
AIT/CYP | - |
+++
|
+
|
Day 7: Control | - |
+
| - |
CYP | - |
+
| - |
AIT/CYP | - |
+
|
+
|
Day 11: Control | - |
++
| - |
CYP | - |
+
| - |
AIT/CYP | - |
+++
|
+
|
- Grading:
- - No stain
- + 1-15%
- ++ 16-50%
- +++ >50%
- Mice with established 4T1 flank tumors were either untreated, treated with CYP alone on day 3, or were treated with CYP on Day 3 after tumor inoculation followed by AIT with 25 × 106 B/I activated 4T1 tDLN (on day 4). At 1, 4, 7 and 11 days after AIT, the tumors were harvested and tumor cross-sections were stained for CD4 and CD8 expression. A representative experiment is shown.