Figure 1

Differentiation of keratinocytes results in a mixture of immature and semi mature cells. Keratinocytes of a representative donor were differentiated in chamber-slides for 6 days. Following fixation, cells were stained for the indicated cytokeratins (CK) using a panel of monoclonal (CK10, CK14, CK16) and polyclonal antibodies (pan). Duration of fluorescence detection was kept constant. Though variable, all cells were positive for CK14, while staining of CK10 and CK16 was limited to areas with blast-like keratinocytes.