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Figure 5 | BMC Immunology

Figure 5

From: T cells fail to develop in the human skin-cell explants system; an inconvenient truth

Figure 5

Keratinocytes are out-competed by fibroblasts. (A) Matrix was stained for keratinocytes and fibroblasts using panCK and CD90, respectively, six days after matrices were seeded at a 2:1 ratio. At this stage, matrices were carefully seeded with HSC. (B) After 4 weeks, matrices were completely covered with CD90+ fibroblasts. HSC-derived cells could only be retrieved from the matrix following collagenase-dispase treatment. Fibroblasts from all donors grew similarly.

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