Figure 5

PD-1-expressing T cells vary in IL21 expression, CXCL13 production, cell proliferation and cell survival abilities. (A) Expression of IL21 mRNA by the T cell subsets. Real time PCR was performed. Shown are expression levels normalized with that of β-actin. (B) CXCL13 production in response to anti-CD3, anti-CD28, and IL-2. (C) Proliferation responses of PD-1-expressing T cells in response to anti-CD3 and anti-CD28. (D) Cell survival rates after 5 days of culture in complete medium in the absence of TCR activators and cytokines. (E) Expression of Ki-67 by PD-1-expressing T cell subsets. Combined data of 3 independent experiments are shown in the graphs. "*" and "**"indicate significant differences from naïve T cells and PD-1⁺⁺ cells respectively.