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Figure 1 | BMC Immunology

Figure 1

From: Regulation of IL-2 gene expression by Siva and FOXP3 in human T cells

Figure 1

FOXP3 physically interacts with both Siva isoforms. A. Diagram showing the Siva protein domain organization (SAH, spherical amphipathic helix; DDHR, death domain homology region; and Zn F, zinc finger) and a cartoon representation of Siva-1 and Siva-2, which were fused to the C-terminus of EGFP. B. Co-immunoprecipitation (Co-IP) to evaluate FOXP3's interaction with Siva-1 and Siva-2 in 293T cells. Lysates from 293T cells transfected with plasmids encoding EGFP/Siva fusion constructs and Myc/FOXP3 or vector were IP'ed with mouse anti-Myc 9e10 hybridoma supernatant. Western blot (WB) was performed with a mouse monoclonal antibody against GFP. Membranes were stripped and reprobed with a rabbit anti-Myc polyclonal antibody. FOXP3 binding to Siva-1 was observed in seven independent experiments; FOXP3 binding to Siva-2 was observed in three out of three experiments. C. Siva expression based on spot intensities for CD4pos T cells isolated from the Foxp3GFP reporter mouse and sorted for both Foxp3GFP and CD25 expression [33]. The data was generated on an Affymetrix Mouse Genome 430 2.0 array platform.

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