Figure 7
From: Regulation of IL-2 gene expression by Siva and FOXP3 in human T cells
IL-2 promoter activity in response to FOXP3 combined with Siva-1 overexpression or Siva knockdown. Similar to the data sets shown above, the IL-2 promoter activity was normalized to viable cell counts. A. IL-2 promoter activity in response to FOXP3 and Siva-1 overexpression. In addition to the IL-2 luciferase reporter plasmid, Jurkat T cells were transfected with different combinations of pHSPG (PG), PG-FOXP3, and PG-Siva-1. B. IL-2 promoter activity in response to FOXP3 overexpression and Siva knockdown. Jurkat T cells were transfected with the IL-2 luciferase reporter, a plasmid containing either a negative control hairpin sequence (shNS) or shSiva, and PG or PG-FOXP3 plasmids (RLUs, relative light units). *s indicate statistically significant difference between the designated bar and cells transfected with either PG in A or shNS and PG in B. Statistically significant difference was based on p < 0.05 by a two-tailed Student's t-test. These experiments were repeated twice and data from one representative experiment is shown.