Figure 1From: Inhibition of TLR8- and TLR4-induced Type I IFN induction by alcohol is different from its effects on inflammatory cytokine production in monocytesDecreased IFNβ levels in monocytes stimulated with acute alcohol and TLR8 or TLR4 ligand. Human monocytes (n = 3) were isolated as described in the methods. Cells were either treated or not with 25 mM ethanol or 2.5 ug/ml CL075 (TLR8 ligand) or 0.1 ug/ml LPS (TLR4 ligand) for 6 hours. Some cells were stimulated with a combination of 25 mM ethanol and 2.5 ug/ml CL075 or 25 mM ethanol and 0.1 ug/ml LPS. The cell-free supernatants were collected and IFNβ production was measured by ELISA. Error bars represent ± SEM. unst: unstimulated, ns: non significant.Back to article page