- Research article
- Open Access
Caries induced cytokine network in the odontoblast layer of human teeth
© Horst et al; licensee BioMed Central Ltd. 2011
Received: 31 August 2010
Accepted: 24 January 2011
Published: 24 January 2011
Immunologic responses of the tooth to caries begin with odontoblasts recognizing carious bacteria. Inflammatory propagation eventually leads to tooth pulp necrosis and danger to health. The present study aims to determine cytokine gene expression profiles generated within human teeth in response to dental caries in vivo and to build a mechanistic model of these responses and the downstream signaling network.
We demonstrate profound differential up-regulation of inflammatory genes in the odontoblast layer (ODL) in human teeth with caries in vivo, while the pulp remains largely unchanged. Interleukins, chemokines, and all tested receptors thereof were differentially up-regulated in ODL of carious teeth, well over one hundred-fold for 35 of 84 genes. By interrogating reconstructed protein interaction networks corresponding to the differentially up-regulated genes, we develop the hypothesis that pro-inflammatory cytokines highly expressed in ODL of carious teeth, IL-1β, IL-1α, and TNF-α, carry the converged inflammatory signal. We show that IL1β amplifies antimicrobial peptide production in odontoblasts in vitro 100-fold more than lipopolysaccharide, in a manner matching subsequent in vivo measurements.
Our data suggest that ODL amplifies bacterial signals dramatically by self-feedback cytokine-chemokine signal-receptor cycling, and signal convergence through IL1R1 and possibly others, to increase defensive capacity including antimicrobial peptide production to protect the tooth and contain the battle against carious bacteria within the dentin.
Cytokines generate and maintain host responses to microbial infection. Living cells of the host secrete these molecules as paracrine or autocrine signals to recruit cells of the immune system (chemokines), produce inflammation (proinflammatory cytokines), or control the inflammatory responses (anti-inflammatory cytokines). The fine-tuned cytokine networks facilitate the eradication of invading microbes but maintain a balance between pro- and anti-inflammation thereby creating a favorable environment for tissue repair .
Dental caries and subsequent tooth pulp inflammation are major oral health problems caused by bacterial infection. Previous studies have reported increased expression of various cytokines in caries-affected dental pulp and/or odontoblasts including transforming growth factor-β1 (TGFβ1), vascular endothelial cell growth factor (VEGF), C-C chemokine ligand 2 (CCL2/MCP1), CCL20/MIP3α, interleukin 8 (IL8/CXCL8), CXC chemokine ligand 10 (CXCL10), epithelial cell-derived neutrophil attractant 78 (ENA78), IL-1β, IL2, IL4, IL6, IL10, IL11, interferon-γ (IFN-γ) and tumor necrotic factor-α (TNF-α) [2–13]. The induction of these cytokines was also shown in cultured pulp-derived fibroblasts and odontoblast-like cells exposed to bacteria or their products in vitro [9, 10, 14–20]. However, these molecular events induced in odontoblast layer (ODL) have not been characterized or distinguished from those of the underlying pulp during the carious process in vivo.
Like osteoblasts and other blast cells, the primary function of odontoblasts is generally understood as producing the extracellular structure of dentin. The functional response of odontoblasts to caries is recognized as extending a barrier to the spread of infection by forming reparative dentin. Recently our group and others showed that odontoblasts can also mediate host inflammatory responses to caries directly through production of antimicrobial peptides and cytokines, and indirectly through activation of migratory immune cells using in vitro and ex vivo models [10, 13–15, 18–20]. In this study we aim to characterize cytokine expression profiles generated within human teeth in response to dental caries in vivo, and to build a mechanistic model of these responses by mapping the in vivo differential gene expression of ODL and pulp in healthy (intact) and diseased (carious) teeth across known protein interactions.
We assessed the tooth inflammatory regulatory network as the combined response of cells in ODL and pulp to dental caries in vivo. First we described gene expression profiling of cytokines and related immune components in ODL and pulp of normal teeth. Second we analyzed expression changes in carious teeth using cDNA microarrays and verified with quantitative real-time PCR. Additional gene transcripts with significant changes in carious teeth were identified by real-time PCR arrays. We mapped the connectivity between differentially expressed gene transcripts onto a network of experimentally demonstrated protein interactions. The reconstructed interaction network between differentially expressed gene products suggested that ODL amplifies responses through self-feedback cycling of signal-receptor events, building the capacity to dramatically increase cytokine and antimicrobial peptide production to protect the tooth and contain the battle against oral pathogens within the dentin.
Gene expression profiling of immune components in the odontoblast layer (ODL) and pulp of normal teeth
Gene expression profile of cytokines and their receptors in the odontoblast layer (ODL) and pulp of normal teeth*.
ODL > PULP
PULP > ODL
BCL6, CEBPB, ICEBERG, IL1R1, IL5RA, IL8, MIF, SPP1, TOLLIP
C3, CCL16, CCL19, CCL2, CCL21, CCL23, CCL25, CCL4, CCL5, CCL8, CCR2, CCR3, CCR5, CX3CR1, CXCL1, CXCL12, CXCL14, CXCL2, CXCL5, IL5, IL9, LTB4R, TNFA, CD40LG, XCR1
In normal teeth, mRNA expression of most genes was higher in the pulp, although expression was higher in ODL for the following genes (Table 1): IL1R1, MIF, toll-interacting protein (TOLLIP), transcription factor 5 or CCAAT/enhancer binding protein beta (CEBPβ/CEBPB), B-cell lymphoma 6 (BCL6), iceberg caspase-1 inhibitor (ICEBERG), IL5Rα/IL5RA, IL8, and osteopentin (OPN/SPP1). C-C family chemokine receptor 3 (CCR3) mRNA was detected in the pulp but not in ODL.
Caries-induced inflammatory gene expression in ODL and underlying pulp
Protective production of antimicrobial peptides induced by pro-inflammatory mediators increased in ODL of carious teeth
We examined the effects of IL-1β/IL1B, TNF-α/TNFA, IFNγ/IFNG, and TLR4 activation on antimicrobial peptide production using in vitro cultured human odontoblast-like cells. The protein products of these genes are major inducers of pulpal inflammation and are well known to regulate production of other cytokines.
Model of caries signal induction
The gene expression analysis findings suggest cell-cell interaction events mediated by pro-inflammatory cytokines, chemokines, and antimicrobial peptides that are predicted to build the immune defense capacity within ODL to protect the tooth and contain the battle against oral pathogens in dentin (Figure 4C).
Mapping of caries-induced molecular interactions for ODL inflammatory responses
A few receptors receive the signal of abundant ligands, and are themselves abundant in ODL of carious teeth including IL1R1, IL8Rα, IL8Rβ, IL9R, IL13Rα1, CCR1, CCR2, CCR3, CCR4, and CCR8. The dramatic increase of either receptors or ligands in ODL (Figure 5A) but not in the pulp (Figure 5B) of carious teeth suggests: 1) signals through these receptors are critical to initiate inflammatory events within ODL, and 2) the propagation of these signals into the pulp may lead to the development of irreversible pulpitis in carious teeth.
Odontoblasts form the peripheral layer of the dental pulp as an internal host-microbial interface for the tooth, and thus are vulnerable and need the capability of innate host defenses [13, 18]. Cytokines and chemokines mediate the crosstalk between odontoblasts and cells of the innate immune system such as neutrophils, monocytes/macrophages, dendritic cells, and natural killer cells. These proteins are secreted by both odontoblasts and immune cells in response to bacterial stimuli to attract additional immune cells as well as initiate and modulate inflammatory responses. Here we propose a mechanistic model of cytokine-signaling network in the odontoblast layer (ODL) of human teeth in response to dental caries and the role of IL1R1 and ligands IL-1β and IL-1α in carrying the converged inflammatory signals to amplify innate immune responses including the production of antimicrobial peptides to protect the tooth and contain the battle against carious bacteria within dentin. We also show that cells in ODL of normal and carious teeth expressed mRNA for various immune components of which the majority measured here are chemotactic cytokines. In response to carious infection, these cytokines are highly up-regulated in ODL and most likely induce leukocyte migration into the tooth to increase immunologic capacity. This finding is supported by previous data in vitro that protein secretions from odontoblast-like cells exposed to bacterial products induced migration of monocyte-derived immature dendritic cells [10, 15].
Our findings of active immune components in healthy teeth expand upon previous findings. One study using healthy teeth reported mRNA expression of TGFα/TGFA, CCR2, CXCL1, and CXCL6 only in ODL, and CCL5, CCL15, and LTB gene expression only in the pulp . Conversely, in this study we found expression of all these markers in both ODL and underlying pulp of normal teeth. Other studies reported mRNA expression of CCL2, CCL26, CXCL12, CXCL14, IL8Rβ/IL8RB, LTB4R, and SCYE1 in cultured human odontoblast-like cells [10, 15], which matches our in vivo results from ODL of normal teeth.
Odontoblasts recognize carious bacteria and initiate immune responses through toll-like receptors (TLRs) [10, 13–15, 18]. TGFβ1 was shown to attenuate odontoblast inflammatory responses by inhibiting TLR2 and TLR4 expression, which maintain homeostasis within the tooth during carious infection . We also noticed other TLR signal antagonists in the tooth including Toll-interacting protein (TOLLIP) and IL10 [22, 23]. High expression of TOLLIP in ODL can provide a negative feedback loop for TLR-mediated inflammation to protect the underlying pulp. IL10 and receptors, IL10Rα/IL10RA and IL10Rβ/IL10RB were present in ODL and pulp. IL10 was highly up-regulated in ODL of carious teeth (>100 fold) and provides another mechanism to attenuate pulp inflammatory responses.
Our hypothesis that ODL is the primary biologic unit of immune responses in the tooth is supported by the profound increase in expression of many inflammatory genes within ODL but not in the pulp. As we do not assess any of the cell types alone, this immune modulatory tissue includes odontoblasts and immune cells such as dendritic cells, macrophages, lymphocytes, and neutrophils. These responses are mediated by cell-to-cell interactions within ODL, and imply differences between in vitro and in vivo responses to carious bacteria. Our findings together suggest that ODL is effective in attenuating carious infections thereby limiting the inflammatory changes within ODL and maintaining the pulp in a relatively protected environment.
In the presence of bacteria, odontoblasts secrete various chemotactic cytokines for neutrophils, monocytes/macrophages, immature dendritic cells, and lymphocytes including interleukin 8 (IL-8), chemokine (C-C motif) ligand 2 (CCL2), CCL7, chemokine (C-X-C motif) ligand 2 (CXCL2), and CXCL10 [10, 13, 24, 25]. Similarly we found up-regulation of these genes in ODL of carious teeth. CXCL2 and CXCL10 mRNA also increased in the pulp tissues of carious teeth but CCL7 slightly decreased.
Other chemokines increased in ODL of carious teeth are CCL1, CCL3-5, CCL8, CCL11, CCL13, CCL15-17, CCL19-21, CCL23-25, CXCL1, CXCL3, CXCL5, CXCL6, CXCL9-11, and CXCL13. The resulting gradient of these chemokines attracts more migration of immune cells into the tooth . The migratory immune cells, in particular monocytes/macrophages, release a large amount of pro-inflammatory cytokines such as IL-1β, TNF-α, IL-6, and IL-12, which regulate inflammatory reactions in the tissue . We previously showed that human odontoblasts increased transcription of pro-inflammatory cytokines, IL-1β and TNF-α in response to bacterial infection in vitro . Here we show that these pro-inflammatory cytokines and others including CRP, ABCF1, IL9, LTA, LTB, IL1A, IL17C, IL1F10, and IL13, were also increased in ODL of carious teeth in vivo.
We attempted to identify candidate signal propagators by mapping caries-induced expression of inflammatory mediators onto an experimentally verified set of protein interactions. Network analysis shows IL1R1 standing out as a possible early amplifier of the caries signal, as one of the most abundantly expressed genes in ODL with or without caries induction (Figure 5A). The well known pro-inflammatory and immunoregulatory cytokine IL1R1 agonists, IL-1α and IL-1β, are both highly expressed by cells in carious ODL. IL-1α is the third most up-regulated gene after ABCF1 and LTA (Figure 5A). The signal propagation from IL1R1 overlaps with the TLR4-activated NFkB pathway, suggesting direct signal amplification (Figure 6).
We demonstrate that activation of IL1R1 by IL-1β (or IL-1α) may carry an important activation signal for innate immune responses, with the example of antimicrobial peptide production (Figure 4). The important role of IL1R1 in protecting the tooth and surrounding bone from polymicrobial infection (Streptococcus mutans, Streptococcus intermedius, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum) was verified in vivo by using genetically modified IL1R1 knockout mice. Pulp tissues of teeth experimentally infected with mixed bacteria became necrotic faster and had greater bacterial invasion in IL1R1-null mice than wild-type controls. Further abscess formation and the loss of surrounding bone around infected teeth were shown to be greater in IL1R1-null mice than wild-type controls [28, 29].
Although cDNA arrays showed a reduction of IL1R1 in ODL of carious teeth, qPCR data indicated that this change was very low and not statistically significant. We also observed similar result for TLR4 expression in ODL of carious teeth (unpublished observation). TLR4 activation amplifies inflammatory signaling through the activation and production of NF-κB, avoidance of ATF3, and cyclic activation of C/EBPδ . While the levels of C/EBPδ, ATF3, and NF-kB control the IL6 output, the supra-threshold level of TLR4 does not affect signal amplification. Moreover, flux in TLR4 production would confuse C/EBPδ interpretation of a transient signal as a persistent signal. We present IL1R1 as playing a similar role in inflammatory signal amplification to that of TLR4.
ABCF1, the most highly upregulated gene in ODL of carious teeth was mapped downstream of TNF-α and caspase 10. This gene is regulated by TNF-α  and cleaved by caspase 10 . Little is known about functions of this gene but it was shown to regulate protein synthesis , inflammatory progression , and apoptosis . Activation of initiator caspases including caspase 8 and 10 during apoptosis could result in the cleavage of ABCF1 and subsequent regulation of apoptotic signaling. The dramatic up-regulation of ABCF1 in ODL of carious teeth may prime the surrounding cells of the ODL for necrosis.
The signaling pathways from TLR4, TGFβ, chemokine, interleukin, and TNF receptors were mediated through various signaling molecules such as MYD88, IKK, TRAF, Smad, MAP kinase, JAK/STAT, and caspases with high interactions and cross-talk among these signaling pathways. Output from this network includes various aggregate cellular responses including the convergence of many pathways onto PIK3R1 (85 kD regulatory subunit of phosphatidylinositol 3-kinase) and PIK3CA (110 kD catalytic subunit of phosphatidylinositol 3-kinase), suggesting that modulation of phosphatidylinositol-3 kinase (PI3K) activity by these proteins could present a mechanism to control the inflammatory responses. Consistent with this hypothesis, inhibition of PI3K in odontoblast-like cells exposed to carious bacteria (i.e Streptococcus mutans) significantly reduced the transcription of inflammatory cytokines IL6 and IL8 .
Cells in the odontoblast layer initiate immunologic responses of the tooth to dental caries through proinflammatory cytokine and chemokine signaling. The model we propose for this cytokine interaction network suggests multiple candidate mediators of signal propagation to irreversible inflammatory damage. The cytokine signaling network reported here provides a map to guide future studies to identify diagnostic or therapeutic targets for pulpal inflammation.
Sample Collection and Preparation
Thirty-two freshly extracted human third molars with complete root formation were collected from patients with consent following an approved protocol of the University of Washington Human Subjects Review Board. Sixteen teeth were intact while the other 16 teeth had moderate to deep caries involving 1/2 to 2/3 of the dentin thickness. The isolation of odontoblast layer (ODL) and underlying pulp was performed as previously described [13, 36, 37].
The periodontal tissues were mechanically removed under a dissecting microscope. The tooth was rinsed with 5.25% NaOCl to ensure complete removal of periodontal tissues. Then the tooth was rinsed with DNase RNase free water and was submerged in phosphate buffered saline (PBS) while a horizontal groove was made 1-2 mm above the roots. The roots were then split off and the loose core of pulp tissue was pulled out, leaving ODL attached to the tooth crown. Both the pulp tissue and crown ODL were placed in RNA Later (Ambion, Austin, Texas) until processed for RNA isolation.
After all pulp and ODL tissues were removed for RNA isolation, the non-decalcified teeth were sectioned into two halves for direct examination of carious infection depth. A spoon excavator was used to remove caries until hard dentin was reached. The depth from the dentin enamel junction to the pulp (dentin thickness) was evaluated in sixths. Teeth with excavation reaching 1/2 to 2/3 of the dentin thickness were selected for use in this study.
Three pooled samples (one from three teeth, another from three teeth, and another from four teeth) were prepared for cDNA array analysis for each tissue group (normal ODL, normal pulp, carious ODL, and carious pulp). Each remaining pooled RNA preparation was analyzed in qPCR verification experiments. Three pooled samples of two teeth each were prepared for the qPCR array for each tissue group. In total, 10 normal teeth (pulp and ODL), and 10 carious teeth (pulp and ODL) were analyzed by cDNA and qPCR verification, and 6 normal teeth (pulp and ODL), and 6 carious teeth (pulp and ODL) were analyzed by PCR arrays.
An in vitro model of human odontoblasts (hOD) was created and characterized in our previous study . Briefly, cells were maintained at 37°C in a 5% CO2 atmosphere in Dulbecco's Modified Eagle's Medium (DMEM)/Nutrient Mixture F-12 without HEPES (Gibco, Grand Island, NY, USA), supplemented with 10% heat-inactivated fetal bovine serum (FBS, Hyclone, Logan, UT, USA), 1 μg/mL of vitamin K1 (Sigma-Aldrich, St. Louis, MO, USA), 50 μg/mL of ascorbic acid (Sigma-Aldrich), 100 I.U./mL of penicillin G, 100 μg/mL of streptomycin sulfate (Gibco), 0.3 μg/mL of fungizone (Gibco), 1% 100X insulin-transferrin-selenium-X (Gibco), and 10 mM β-glycerophosphate (Sigma-Aldrich). A library of in vitro odontoblast-like cell clones was established. Each clone was grown from a single cell. From previously described clones , the hOD2 clone was chosen because expression of odontoblast markers DSPP and DMP1 is relatively similar to native human odontoblasts, and higher than other clones we evaluated. The hOD2 cells were stimulated with each of the three pro-inflammatory cytokines IL-1β (100 ng/ml, Catalog# PHC0814, BioSource, Invitrogen, Carlsbad, CA), TNF-α (100 ng/ml, Catalog# CRT100A, Cell Sciences, Canton, MA), IFNγ (100 ng/ml, Catalog# CRI000A, Cell Sciences) or TLR4 agonist (100 ng/ml, Purified E. coli LPS). Specificity of TLR4 activation by E. coli LPS used in this study was verified in our previous study .
RNA Isolation and cDNA Microarray
Total RNA of ODL and pulp was separately extracted by using Trizol® Reagent (Invitrogen, Carlsbad, CA), treated with RNase-free DNase (Qiagen, Valencia, CA), and purified by using RNeasy® minikit (Qiagen).
Total RNA was isolated from cultured cells and purified using similar methods. To verify adequate RNA quality, RNA integrity numbers of all samples were confirmed to be more than 7.0 by using Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). The cDNA arrays (GEArray™ Q series human inflammatory cytokines and receptors HS-015) were processed and analyzed according to the manufacturer's instruction (SA Biosciences, Frederick, MD) and as previously described [10, 38, 39].
Briefly, each cDNA probe was synthesized from 1.5 μg of total RNA using RT-Labeling kit (SA Biosciences, Frederick, MD). Then the cDNA probe was labeled with biotin using the AmpoLabeling-LPR kit (SA Biosciences) according to the manufacturer's instruction. The cDNA probe was hybridized onto each array membrane (GEArray™ Q series human inflammatory cytokines and receptors HS015, SA Biosciences). The chemiluminescent signals on each array were detected by exposing the array membrane onto an X-ray film, which was processed, scanned, and saved into TIFF image file. The chemiluminescent signals were quantified and analyzed by using the SA Bioscience microarray software analysis suite. The analyses were performed on a triplicate set of cDNA arrays using three different pooled samples of normal ODL, normal pulp, carious ODL, and carious pulp.
The genes for cytokines and receptors included in the cDNA arrays are BLR1, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, XCR1, CX3CR1, CXCR4, IFNG, IL10, IL10RA, IL10RB, IL11, IL11RA, IL12A, IL12B, IL12RB1, IL12RB2, IL13, IL13RA1, IL13RA2, IL15, IL15RA, IL16, IL17A, IL17RA, IL18, IL18R1, IL1A, IL1B, IL1R1, IL1R2, IL2, IL20, IL21, IL25, IL2RA, IL2RB, IL2RG, IL4, IL5, IL5RA, IL6, IL6R, IL6ST, IL9, IL9R, LEP, LTA, LTB, LTBR, MIF, CCL1, CCL11, CCL13, IL3, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL3, CCL4, CCL5, CCL7, CCL8, CXCL10, CXCL11, CXCL13, CXCL5, CXCL6, XCL1, XCL2, CX3CL1, SCYE1, CXCL12, SDF2, TGFA, TGFB1, TGFB2, TGFB3, TNFA, TNFRSF1A, TNFRSF1B.
Quantitative Real-Time Polymerase Chain Reaction (qPCR)
PCR reagents and all PCR primers for cytokines and receptors except those for detecting β-defensin genes were purchased from SA Biosciences. Specific primers for human β-defensin 1 (HBD1), HBD2, HBD3, and house keeping genes GAPDH are as follows: HBD1 sense, 5"- CAC TTG GCC TTC CCT CTG TA-3"; HBD1 antisense, 5"-CGC CAT GAG AAC TTC CTA CC-3"; HBD2 sense, 5'-CCA GCC ATC AGC CAT GAG GGT-3'; HBD2 antisense, 5'-GGA GCC CTT TCT GAA TCC GCA-3"; HBD3 sense, 5"-GTG AAG CCT AGC AGC TAT GAG GAT-3"; HBD3 antisense, 5"- TGA TTC CTC CAT GAC CTG GAA-3"; GAPDH sense, 5"-CAA AGT TGT CAT GGA TGA CC -3"; and GAPDH antisense, 5"-CCA TGG AGA AGG CTG GGG-3".
The quantitative real-time PCR (qPCR) amplification was performed using the iCycler system (Bio-Rad, Hercules, CA). Briefly, 1 μg of total RNA was used for cDNA synthesis using RT2 First Strand kit (SA Biosciences). 1 μl of the resulting cDNA product was used for each PCR reaction.
Each PCR reaction contains 12.5 μl of RT2 SYBR Green qPCR Master Mixes (PA-011, SA Biosciences), 10.5 μl of nuclease-free water, 1 μl of cDNA, and 1 μl of 10 μM PCR primers. Thermocycling conditions were initial denaturation at 95°C for 10 min and amplification at 40 cycles of 95°C for 15 sec, followed by 60°C for 1 min. PCR analyses were performed in triplicates and repeated three times using three different pooled samples. GAPDH was used as house keeping gene. In initial experiments, amplification efficiency was determined for all primer pairs. Melt curve analysis confirmed a single specific PCR product from each primer pair. The threshold cycle number was determined on the amplification plot during the early log phase of product accumulation at which the fluorescence clearly rises above background in a straigth line. Quantification was performed using the comparative Ct method as previously described , using the amplification efficiency determined for each primer pair, and that compared to GAPDH.
For PCR arrays (PAHS-011: Human Inflammatory Cytokines & Receptors PCR Array, SA Biosciences), 91 μl of nuclease free water was added into 20 μl of the resulting cDNA product to make 111 μl volume. Then 102 μl of the cDNA was used for 96 PCR reactions containing 1150 μl of RT2 SYBR Green qPCR Master Mixes (PA-011, SA Biosciences), and 1048 μl of nuclease-free water. 20 μl of this mixture was loaded into each well of a PCR array plate containing each specific PCR primer pair. Thermocycling conditions were initial denaturation at 95°C for 10 min and amplification at 40 cycles of 95°C for 15 sec, followed by 60°C for 1 min. PCR array analyses were performed in triplicates using three different pools of each sample type. Relative fold changes of gene expression were calculated using the Delta-Delta Ct quantification method (Web-Based PCR Array Data Analysis software, SA Biosciences).
The genes for cytokines and receptors included in the PCR arrays are ABCF1, BCL6, C3, C4A, C5, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL23, CCL24, CCL25, CCL26, CCL3, CCL4, CCL5, CCL7, CCL8, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CEBPB, CRP, CX3CR1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL14, CXCL2, CXCL3, CXCL5, CXCL6, CXCL9, ICEBERG, IFNA2, IL10, IL10RA, IL10RB, IL13, IL13RA1, IL17C, IL1A, IL1B, IL1F10, IL1F5, IL1F6, IL1F7, IL1F8, IL1F9, IL1R1, IL1RN, IL22, IL5, IL5RA, IL8, IL8RA, IL8RB, IL9, IL9R, LTA, LTB, LTB4R, MIF, SCYE1, SPP1, TNFA, CD40LG, TOLLIP, XCR1.
Protein Interaction Mapping
We built a protein-protein interaction map to model the molecular mechanisms of any biological process induced by proteins encoded by genes significantly increased in response to dental caries.
The list of 35 unconnected genes excluded from the protein-protein interaction map.
C4A, CRP, LTB, LTB4R, CXCL2, CXCL3, CXCL9, CXCL10, CXCL11, CXCL12, CXCL13, CXCL14, CCL15, CCL18, CCL19, CCL20, CCL21, CCL25, XCR1, CX3CR1, CCR6, CCR7, CCR9, IL17C, IL1F5, IL1F6, IL1F7, IL1F8, IL1F9, IL1F10, SPP1, ICEBERG, BCL6, SCYE1, MIF
The data were analyzed using the analysis of variance followed by the Tukey test for multiple comparisons. Results were considered statistically significant when the P-value was less than 0.05.
This study was supported by T32DE11894 & F30DE17522.
- O'Shea JJ, Ma A, Lipsky P: Cytokines and autoimmunity. Nat Rev Immunol. 2002, 2: 37-45.PubMedView ArticleGoogle Scholar
- Adachi T, Nakanishi T, Yumoto H, Hirao K, Takahashi K, Mukai K, Nakae H, Matsuo T: Caries-related bacteria and cytokines induce CXCL10 in dental pulp. J Dent Res. 2007, 86: 1217-1222. 10.1177/154405910708601215.PubMedView ArticleGoogle Scholar
- Artese L, Rubini C, Ferrero G, Fioroni M, Santinelli A, Piattelli A: Vascular endothelial growth factor (VEGF) expression in healthy and inflamed human dental pulps. J Endod. 2002, 28: 20-23. 10.1097/00004770-200201000-00005.PubMedView ArticleGoogle Scholar
- Kokkas AB, Goulas A, Varsamidis K, Mirtsou V, Tziafas D: Irreversible but not reversible pulpitis is associated with up-regulation of tumour necrosis factor-alpha gene expression in human pulp. Int Endod J. 2007, 40: 198-203. 10.1111/j.1365-2591.2007.01215.x.PubMedView ArticleGoogle Scholar
- McLachlan JL, Sloan AJ, Smith AJ, Landini G, Cooper PR: S100 and cytokine expression in caries. Infect Immun. 2004, 72: 4102-4108. 10.1128/IAI.72.7.4102-4108.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- McLachlan JL, Smith AJ, Bujalska IJ, Cooper PR: Gene expression profiling of pulpal tissue reveals the molecular complexity of dental caries. Biochim Biophys Acta. 2005, 1741: 271-281.PubMedView ArticleGoogle Scholar
- Piattelli A, Rubini C, Fioroni M, Tripodi D, Strocchi R: Transforming growth factor-beta 1 (TGF-beta 1) expression in normal healthy pulps and in those with irreversible pulpitis. Int Endod J. 2004, 37: 114-119. 10.1111/j.0143-2885.2004.00758.x.PubMedView ArticleGoogle Scholar
- Rauschenberger CR, Bailey JC, Cootauco CJ: Detection of human IL-2 in normal and inflamed dental pulps. J Endod. 1997, 23: 366-370. 10.1016/S0099-2399(97)80184-7.PubMedView ArticleGoogle Scholar
- Hahn CL, Best AM, Tew JG: Cytokine induction by Streptococcus mutans and pulpal pathogenesis. Infect Immun. 2000, 68: 6785-6789. 10.1128/IAI.68.12.6785-6789.2000.PubMedPubMed CentralView ArticleGoogle Scholar
- Durand SH, Flacher V, Romeas A, Carrouel F, Colomb E, Vincent C, Magloire H, Couble ML, Bleicher F, Staquet MJ, et al: Lipoteichoic acid increases TLR and functional chemokine expression while reducing dentin formation in in vitro differentiated human odontoblasts. J Immunol. 2006, 176: 2880-2887.PubMedView ArticleGoogle Scholar
- Sloan AJ, Perry H, Matthews JB, Smith AJ: Transforming growth factor-beta isoform expression in mature human healthy and carious molar teeth. Histochem J. 2000, 32: 247-252. 10.1023/A:1004007202404.PubMedView ArticleGoogle Scholar
- Nakanishi T, Takahashi K, Hosokawa Y, Adachi T, Nakae H, Matsuo T: Expression of macrophage inflammatory protein 3alpha in human inflamed dental pulp tissue. J Endod. 2005, 31: 84-87. 10.1097/01.don.0000143414.22112.57.PubMedView ArticleGoogle Scholar
- Veerayutthwilai O, Byers MR, Pham TT, Darveau RP, Dale BA: Differential regulation of immune responses by odontoblasts. Oral Microbiol Immunol. 2007, 22: 5-13. 10.1111/j.1399-302X.2007.00310.x.PubMedView ArticleGoogle Scholar
- Botero TM, Shelburne CE, Holland GR, Hanks CT, Nor JE: TLR4 mediates LPS-induced VEGF expression in odontoblasts. J Endod. 2006, 32: 951-955. 10.1016/j.joen.2006.03.018.PubMedView ArticleGoogle Scholar
- Staquet MJ, Durand SH, Colomb E, Romeas A, Vincent C, Bleicher F, Lebecque S, Farges JC: Different roles of odontoblasts and fibroblasts in immunity. J Dent Res. 2008, 87: 256-261. 10.1177/154405910808700304.PubMedView ArticleGoogle Scholar
- Takahashi K, Nakanishi T, Yumoto H, Adachi T, Matsuo T: CCL20 production is induced in human dental pulp upon stimulation by Streptococcus mutans and proinflammatory cytokines. Oral Microbiol Immunol. 2008, 23: 320-327. 10.1111/j.1399-302X.2008.00431.x.PubMedView ArticleGoogle Scholar
- Tokuda M, Sakuta T, Fushuku A, Torii M, Nagaoka S: Regulation of interleukin-6 expression in human dental pulp cell cultures stimulated with Prevotella intermedia lipopolysaccharide. J Endod. 2001, 27: 273-277. 10.1097/00004770-200104000-00008.PubMedView ArticleGoogle Scholar
- Horst OV, Tompkins KA, Coats SR, Braham PH, Darveau RP, Dale BA: TGF-beta1 Inhibits TLR-mediated odontoblast responses to oral bacteria. J Dent Res. 2009, 88: 333-338. 10.1177/0022034509334846.PubMedPubMed CentralView ArticleGoogle Scholar
- Farges JC, Keller JF, Carrouel F, Durand SH, Romeas A, Bleicher F, Lebecque S, Staquet MJ: Odontoblasts in the dental pulp immune response. J Exp Zool B Mol Dev Evol. 2009, 312B: 425-436. 10.1002/jez.b.21259.PubMedView ArticleGoogle Scholar
- Keller JF, Carrouel F, Colomb E, Durand SH, Baudouin C, Msika P, Bleicher F, Vincent C, Staquet MJ, Farges JC: Toll-like receptor 2 activation by lipoteichoic acid induces differential production of pro-inflammatory cytokines in human odontoblasts, dental pulp fibroblasts and immature dendritic cells. Immunobiology. 2010, 215: 53-59. 10.1016/j.imbio.2009.01.009.PubMedView ArticleGoogle Scholar
- Paakkonen V, Vuoristo JT, Salo T, Tjaderhane L: Comparative gene expression profile analysis between native human odontoblasts and pulp tissue. Int Endod J. 2008, 41: 117-127.PubMedGoogle Scholar
- Chang J, Kunkel SL, Chang CH: Negative regulation of MyD88-dependent signaling by IL-10 in dendritic cells. Proc Natl Acad Sci USA. 2009, 106: 18327-18332. 10.1073/pnas.0905815106.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang G, Ghosh S: Negative regulation of toll-like receptor-mediated signaling by Tollip. J Biol Chem. 2002, 277: 7059-7065. 10.1074/jbc.M109537200.PubMedView ArticleGoogle Scholar
- Levin LG, Rudd A, Bletsa A, Reisner H: Expression of IL-8 by cells of the odontoblast layer in vitro. Eur J Oral Sci. 1999, 107: 131-137. 10.1046/j.0909-8836.1999.eos107209.x.PubMedView ArticleGoogle Scholar
- Staquet MJ, Durand SH, Colomb E, Romeas A, Vincent C, Bleicher F, Lebecque S, Farges JC: Different Roles of Odontoblasts and Fibroblasts in Immunity. J Dent Res. 2008, 87: 256-261. 10.1177/154405910808700304.PubMedView ArticleGoogle Scholar
- Silva TA, Garlet GP, Fukada SY, Silva JS, Cunha FQ: Chemokines in oral inflammatory diseases: apical periodontitis and periodontal disease. J Dent Res. 2007, 86: 306-319. 10.1177/154405910708600403.PubMedView ArticleGoogle Scholar
- Curfs JH, Meis JF, Hoogkamp-Korstanje JA: A primer on cytokines: sources, receptors, effects, and inducers. Clin Microbiol Rev. 1997, 10: 742-780.PubMedPubMed CentralGoogle Scholar
- Graves DT, Chen CP, Douville C, Jiang Y: Interleukin-1 receptor signaling rather than that of tumor necrosis factor is critical in protecting the host from the severe consequences of a polymicrobe anaerobic infection. Infect Immun. 2000, 68: 4746-4751. 10.1128/IAI.68.8.4746-4751.2000.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen CP, Hertzberg M, Jiang Y, Graves DT: Interleukin-1 and tumor necrosis factor receptor signaling is not required for bacteria-induced osteoclastogenesis and bone loss but is essential for protecting the host from a mixed anaerobic infection. Am J Pathol. 1999, 155: 2145-2152.PubMedPubMed CentralView ArticleGoogle Scholar
- Litvak V, Ramsey SA, Rust AG, Zak DE, Kennedy KA, Lampano AE, Nykter M, Shmulevich I, Aderem A: Function of C/EBPdelta in a regulatory circuit that discriminates between transient and persistent TLR4-induced signals. Nat Immunol. 2009, 10: 437-443. 10.1038/ni.1721.PubMedPubMed CentralView ArticleGoogle Scholar
- Richard M, Drouin R, Beaulieu AD: ABC50, a novel human ATP-binding cassette protein found in tumor necrosis factor-alpha-stimulated synoviocytes. Genomics. 1998, 53: 137-145. 10.1006/geno.1998.5480.PubMedView ArticleGoogle Scholar
- Bae S, Ha TS, Yoon Y, Lee J, Cha HJ, Yoo H, Choe TB, Li S, Sohn I, Kim JY, et al: Genome-wide screening and identification of novel proteolytic cleavage targets of caspase-8 and -10 in vitro. Int J Mol Med. 2008, 21: 381-386.PubMedGoogle Scholar
- Tyzack JK, Wang X, Belsham GJ, Proud CG: ABC50 interacts with eukaryotic initiation factor 2 and associates with the ribosome in an ATP-dependent manner. J Biol Chem. 2000, 275: 34131-34139. 10.1074/jbc.M002868200.PubMedView ArticleGoogle Scholar
- Ota M, Katsuyama Y, Hamano H, Umemura T, Kimura A, Yoshizawa K, Kiyosawa K, Fukushima H, Bahram S, Inoko H, Kawa S: Two critical genes (HLA-DRB1 and ABCF1)in the HLA region are associated with the susceptibility to autoimmune pancreatitis. Immunogenetics. 2007, 59: 45-52. 10.1007/s00251-006-0178-2.PubMedView ArticleGoogle Scholar
- Dommisch H, Steglich M, Eberhard J, Winter J, Jepsen S: Phosphatidylinositol-3-kinase inhibitor LY 294002 blocks Streptococcus mutans-induced interleukin (IL)-6 and IL-8 gene expression in odontoblast-like cells. Int Endod J. 2008, 41: 763-771. 10.1111/j.1365-2591.2008.01429.x.PubMedView ArticleGoogle Scholar
- Tjaderhane L, Salo T, Larjava H, Larmas M, Overall CM: A novel organ culture method to study the function of human odontoblasts in vitro: gelatinase expression by odontoblasts is differentially regulated by TGF-beta1. J Dent Res. 1998, 77: 1486-1496. 10.1177/00220345980770070301.PubMedView ArticleGoogle Scholar
- McLachlan JL, Smith AJ, Sloan AJ, Cooper PR: Gene expression analysis in cells of the dentine-pulp complex in healthy and carious teeth. Arch Oral Biol. 2003, 48: 273-283. 10.1016/S0003-9969(03)00003-7.PubMedView ArticleGoogle Scholar
- Bettaccini AA, Baj A, Accolla RS, Basolo F, Toniolo AQ: Proliferative activity of extracellular HIV-1 Tat protein in human epithelial cells: expression profile of pathogenetically relevant genes. BMC Microbiol. 2005, 5: 20-10.1186/1471-2180-5-20.PubMedPubMed CentralView ArticleGoogle Scholar
- Hastings JM, Jackson KS, Mavrogianis PA, Fazleabas AT: The estrogen early response gene FOS is altered in a baboon model of endometriosis. Biol Reprod. 2006, 75: 176-182. 10.1095/biolreprod.106.052852.PubMedView ArticleGoogle Scholar
- Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29: e45-10.1093/nar/29.9.e45.PubMedPubMed CentralView ArticleGoogle Scholar
- Jensen LJ, Kuhn M, Stark M, Chaffron S, Creevey C, Muller J, Doerks T, Julien P, Roth A, Simonovic M, et al: STRING 8--a global view on proteins and their functional interactions in 630 organisms. Nucleic Acids Res. 2009, 37: D412-416. 10.1093/nar/gkn760.PubMedPubMed CentralView ArticleGoogle Scholar
- Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T: Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 2003, 13: 2498-2504. 10.1101/gr.1239303.PubMedPubMed CentralView ArticleGoogle Scholar
- Kanehisa M, Goto S: KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000, 28: 27-30. 10.1093/nar/28.1.27.PubMedPubMed CentralView ArticleGoogle Scholar
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