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Figure 2 | BMC Immunology

Figure 2

From: TGFβ signaling plays a critical role in promoting alternative macrophage activation

Figure 2

Expression of M2 markers on BMDMs is impaired in the absence of TβRII signaling. (A) Western blot analysis of WT and TβRII-/- BMDMs cultured for 24 hrs with medium alone (M0), with LPS/IFNγ (M1), IL-4 (M2), or hTGFβ1. β-actin serves as a loading control. Increased Arg-1 in KO BMDM upon hTGFβ1stimulation due to residual WT cells, see Additional file 1: Figure S2E. (B) Real-time RT-PCR for the indicated genes after incubation for 24 hrs with medium alone (M0) or IL-4 (M2). (C) Real-time RT-PCR for the indicated genes in naïve peritoneal macrophages. Fold change is with respect to the expression level of M0 WT. Solid bar, WT; Open bar, TβRII-/- peritoneal macrophages. The results shown are representative of one of two independently derived sets of BMDMs from different mice.

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