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Figure 3 | BMC Immunology

Figure 3

From: Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer higher specificity to serodiagnostic assays than whole mite extract

Figure 3

Reaction of anti- B. tropicalis , recombinant allergen IgE antibodies and total IgE to A. lumbricoides extract. Twenty adults' sera with anti-B. tropicalis extract IgE antibodies were pre-incubated with the indicated concentrations of A. lumbricoides crude extract and tested in indirect ELISAs using B. tropicalis crude extract (BtE) or recombinant allergens (rBlo t 5 and rBlo t 21) as antigens or assayed for total IgE levels. The reductions in anti-Bt, anti-rBlo t 5, and anti-rBlo t 21 IgE antibody levels or total IgE levels were calculated as described in the Methods section (A), * p < 0.0231, ** p < 0.0020 and *** p < 0.0008; Wilcoxon signed rank test. (B) Specific sIgE levels in sera non-adsorved and adsorved with Ascaris lumbricoides extract (OD 450 nm), * p < 0.0217 ** p < 0.0012, # p < 0.0030, ## p< 0.0041; Wilcoxon signed rank test.

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