Figure 4
From: Proton channel HVCN1 is required for effector functions of mouse eosinophils
Enhanced cell death in HVCN1-deficient eosinophils following PMA stimulation. (A) The viable eosinophils at the indicated time points following PMA (50 ng/ml) stimulation were stained with trypan blue and counted. Data are expressed as the mean ± SD of 3 separate experiments performed in duplicate. (B) Analysis of eosinophil apoptosis / cell death after four-hour PMA (50 ng/ml) stimulation by flow cytometer Calibur I. Data are expressed as the mean ± SD of 4 separate experiments. (C) Kinetic analysis of eosinophil apoptosis / cell death following PMA (50 ng/ml) stimulation. (D)Analysis of eosinophil apoptosis / cell death after four-hour anisomycin (10 μM) or anti-Fas antibody (1 μg/ml) stimulation by flow cytometer Canto II. Data are expressed as the mean ± SD of 3 separate experiments. *, P < 0.05; **, P < 0.01; n.s., not significant.