Table 2 Primers for real time PCR
From: Characterization of murine macrophages from bone marrow, spleen and peritoneum
Gene | Sequences of primers | |
|---|---|---|
β-actin | Left:5′-GATTACTGCTCTGGCTCCTAG-3’ | Right:5′-GCCACCGATCCACACAGAGT-3’ |
IL-10 | Left:5′-CCAGTACAGCCGGGAGACA-3’ | Right:5′-CAGCTGGTCCTTTGTTTGAAAG-3’ |
TGF-β | Left:5′-TTAGGAAGGACCTGGGTTGG-3’ | Right:5′-AGGGCAAGGACCTTGCTGTA-3’ |
IL-6 | Left:5′-CACAAGTCCGGAGAGGAGAC-3’ | Right:5′-TTGCCATTGCACAACTCTTT-3’ |
IL-12 | Left:5′-GACATCACACGGGACCAAAC-3’ | Right:5′-TACCAAGGCACAGGGTCATC-3’ |
TNF-α | Left:5′-TGCCTATGTCTCAGCCTCTTC-3' | Right: 5′-GAGGCCATTTGGGAACTTCT-3' |