Figure 4

Characterization of human alveolar macrophages (AMΦ) after endobronchial allergen provocation in healthy individuals and asthmatics. AMΦ were isolated at baseline (B) and from saline (S), standard allergen (A) and one-tenth of the standard allergen dose (A1/10) challenged segments. A, Protein surface marker expression of AMΦ, mean fluorescence intensities (MFI) after isotype control correction are shown (n = 4 for healthy subjects and n = 6-8 for asthmatics). B, mRNA expression of CCL13, CCL17 and CLEC10A as fold induction compared to individual baseline control (n = 7). C, Correlation between CCL17 and CLEC10A mRNA expression and eosinophilia in BAL of allergen challenged segments (A + A1/10, n = 14). D, Gene expression under baseline conditions (as fold induction compared to mean baseline expression in macrophages of healthy subjects) (n = 7). Data (A, and B) were analyzed with one way ANOVA. *P < .05, ** P < .01 **, and *** P < .001. The Spearman rank correlation test was used in C.