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Figure 7 | BMC Immunology

Figure 7

From: Flt3+ macrophage precursors commit sequentially to osteoclasts, dendritic cells and microglia

Figure 7

Differentiation potential of FL cells is time dependent Day 6-, day 8-, and day 11-FL cells were differentiated to osteoclasts, DCs, and microglia using specific cytokine combinations. Percentage of differentiated cells was measured as specified below. (A) Cells were grown in presence of M-CSF and RANKL and the number of osteoclasts was counted 6 days later. Similar results have been consistently obtained in several experiments. (B) Cells were treated by GM-CSF and mature DC percentage was determined by flow cytometry determination of CD11c+ cells. Results are representative of 3 independent experiments. (C) Microglia differentiation was assessed by detection of ramified cells after cultivating the cells for 6 days in the presence of GCCM. This experiment is representative of 4 independent experiments.

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