Figure 1
From: Enhanced immunogenicity of a functional enzyme by T cell epitope modification
Guinea pig antigen-specific IgG responses to B. lentus subtilisin and BPN' Y217L. Two groups of four Hartley Guinea pigs were immunized with 20 μg of protease five times over a 10-week period. Serum samples were taken every two weeks. Antibody titers were determined by ELISA. Titers were calculated by determining the ODs at 405 nm where y = 0.5, using a regression analysis of titer data for each animal. Figure 1A shows the average titers and standard deviations in a log base 2 format, Figure 1B represents the same data in a base 10 format.