Figure 1

Amino acid alignment of deer mouse (Pema), house mouse (Mus), rat (Rattus) and human (Homo) IL-2. The house mouse, rat and human sequences were truncated to align with the cloned deer mouse fragment. The sequence accession numbers are listed in Table 3 and were aligned using the clustalw multiple alignment algorithm in MacVector DNA analysis software. Identical amino acids are boxed with dark background, similar amino acids are boxed with light background, and nonidentities have white background. The deer mouse polypeptide represents all but the final 3 amino acids. An unusual feature of house mouse IL-2 is the presence of a serine- and glutamine-rich region near the N-terminus. A conserved threonine (+) that is a potential glycosylation site is located at position 3. All three cysteine residues are conserved in deer mouse IL-2 and it is predicted that a disulfide bond is formed between C72 and C120 (*). Conserved residues involved in receptor binding are located at positions 20 and 42 (^). The predicted alpha helices are denoted by lettered (A-F) underlines.