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Figure 2 | BMC Immunology

Figure 2

From: Analysis of the CCR3 promoter reveals a regulatory region in exon 1 that binds GATA-1

Figure 2

Binding of transcription factors to CCR3 exon 1. In (A), a schematic representation of the CCR3 exon region used for electrophoretic mobility shift assays is shown. E1-FL is the exon 1 full-length probe. Overlapping short probes are called E1-A, E1-B and E1-C, respectively. The DNA sequence is shown with GATA sites boxed. In (B), EMSA assay with the full-length probe and extracts from eosinophilic AML14.3D10 cells is shown. As cold competitors (CC), the full-length probe and short probes E1-A through C were used. The arrow depicts the specific band. A representative experiment, of three similar experiments, is shown.

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