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Table 1 Comparison of PBMC cytokine secretion using central versus peripheral laboratory study models

From: Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study

 

IL-5a

IL-13a

TNFa

IFN-γa

IL-10a

IL-8b

IL-12a

PHA

       

- Peripheral lab (fresh)

26 (8.7)

422* (1.9)

4,400* (3.0)

73,000* (1.4)

557* (0.9#)

  

- Central lab (cryopreserved)

19 (8.0)

252* (3.3)

2,100* (2.0)

15,000* (4.0)

38* (7.1#)

  

LPS

       

- Peripheral lab (fresh)

  

2,600* (1.3)

68 (10)

981* (2.7)

332* (1.9)

7.9 (9.9#)

- Central lab (cryopreserved)

  

1,600* (5.2)

117(15)

708* (3.1)

141* (1.9)

6.6 (24#)

TT

       

- Peripheral lab (fresh)

8.2 (6.0)

555 (5.3)

493* (13)

81,400* (8.8)

24* (3.1#)

  

- Central lab (cryopreserved)

7.2 (26)

714 (2.5)

103* (1.4)

10,900* (1.6)

7* (20#)

  
  1. Values are geometric mean (apg/ml, bng/ml); Percent coefficient of variation shown in parentheses. Red color and * indicates <0.01 for difference in cytokine produced between fresh v. cryopreserved PBMC; Blue color and # indicates <0.05 for difference in variance