Figure 2

Flow cytometry of major spleen leukocyte populations in CCDWT and CCDD. Spleen cells were collected from chimaeras at least three months post-reconstitution, and stained for various lineage markers as described in Materials and Methods. One representative staining of at least 6 separate experiments on different mice of each chimaera type is shown. The % of cells staining for each type is shown above the marker (histograms), in each quadrant (e) or in the gate shown (d, f). An example of isotype control staining is shown in right column of panel d. a) Forward/side scatter plot for spleen cells showing normal distribution of size/granularity in both CCDWT and CCDD. Gate shows region analysed for plots b-f b) Single colour staining for B cells with anti-B220 antibody. c) Single colour staining for T cells with anti-CD3 antibody. d) Two colour staining for macrophages using anti-F4/80 and antibody anti-CD11b. e) Two colour staining with anti-CD4 and anti-CD8 antibodies. f) Two colour staining for dendritic cells with anti-CD11b and anti-CD11c antibodies.