Figure 6
From: Haematopoietic development and immunological function in the absence of cathepsin D
Antibody responses to soluble and particulate antigens in CCDWT and CCDD. a) ELISA from chimaeric mice immunised with 6 μg DNP-BIg/alum as described in the text. IgM responses were measured five days post priming; the highest concentration of plasma corresponds to a dilution of 1: 30. IgG1 and IgG 2b responses were measured six days post boost; the highest concentration of plasma corresponds to a dilution of 1: 100. Each point represents average and standard error of the mean of three mice. Preimmune sera all gave OD values of less than 0.2 at the highest concentration shown. b) ELISA from plasma of chimaeric mice immunised with SRBC (108 cells/mouse). IgM responses were measured five days post priming; the highest concentration of plasma corresponds to a dilution of 1: 300. IgG1 and IgG 2b responses were measured six days post boost; the highest concentration of plasma corresponds to a dilution of 1: 900. Each point represents average of at least six mice. Preimmune sera all gave OD values of less than 0.1 at the highest concentration shown.