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Figure 4 | BMC Immunology

Figure 4

From: Production of monoclonal antibodies reactive with ovine eosinophils

Figure 4

Immunocytochemistry of ovine tissues stained with eosinophil-specific mAbs. Tissue-infiltrating eosinophils were observed in frozen tissue sections following H&E staining in (A) lung tissue collected 48 h post-HDM allergen challenge, and (B) abomasum and (C) associated lymph node taken from a parasite-infected sheep. Immunoperoxidase staining of near serial tissue sections of lung, abomasum and lymph node with (D, E, F) mAb 1.2 and (G, H, I) mAb 3.252 shows staining associated with infiltrating eosinophils in each of the tissues examined (see arrows). Note the diffuse brown positive staining observed with mAb 1.2 compared with the more distinct reactivity of mAb 3.252. Higher magnification insets (in D and G) show detail of positively-stained eosinophils in allergen-challenged lung tissues.

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