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Figure 1 | BMC Immunology

Figure 1

From: Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity

Figure 1

Map and characterization of vaccine constructs. (A) Expression cassettes of the indicated vaccine constructs are shown. All open reading frames are under the control of the human cytomegalovirus immediate early promoter (CMV) or its derivative (CMVtetO2). His6: C-terminal tag; L-GM: leader peptide of GM-CSF; m: murine; rh: rhesus monkey; "": [Gly4Ser]3-linker. (B) Ovalbumin-specific Western Blot analysis of supernatants of 293T cells transiently transfected with plasmids containing the indicated expression cassettes. GFP: supernatant of cells transfected with a GFP expression plasmid. (C) GM-CSF dependent FDCP-1 proliferation. 293 cells were infected with adenoviral vectors containing the indicated expression cassettes and the GM-CSF levels in the supernatants were determined 24 h post infection. Serial dilutions of the supernatants were co-cultured with FDCP-1 cells for 2 days. Proliferation was measured by a MTT assay.

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BMC Immunology

ISSN: 1471-2172

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