Skip to main content
Figure 6 | BMC Immunology

Figure 6

From: Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity

Figure 6

GM-CSF does not influence the activation status of CD8+ lymphocytes. Mice were immunized as described in figure legend 5. The effector phenotype of the antigen-specific CD8+ cells was confirmed by costaining for CD8, SIINFEKL/H-2Kb, and CD62L. CD8 and Tetramer double positive cells were gated (A) and analysed for CD62L expression (B, D). D) Mean percentage and SEM of CD62L-low cells from the CD8 and Tetramer double positive cells from three mice per group are shown. Panel C gives a representative example of CD62L expression levels of the CD8+, but tetramer negative population.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us