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Figure 8 | BMC Immunology

Figure 8

From: Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity

Figure 8

GM-OVA induces neutralizing antibodies against murine GM-CSF. Mice were immunized with the indicated DNA prime adenoviral vector boost regimens. One week after the adenoviral vector booster immunization, the GM-CSF neutralizing activity was determined on the GM-CSF dependent FDCP-1 cell line stimulated with 2,5 ng murine GM-CSF/ml). Mouse sera were tested in a 1/20 (white bars) and a 1/100 (black bars) dilution. Medium without GM-CSF served as a negative control, whereas GM-CSF-containing medium without mouse serum was the positive control.

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BMC Immunology

ISSN: 1471-2172

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