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Figure 7 | BMC Immunology

Figure 7

From: Dendritic Cells are preferentially targeted among hematolymphocytes by Modified Vaccinia Virus Ankara and play a key role in the induction of virus-specific T cell responses in vivo

Figure 7

MVA-infected DCs induced Ag-specific CTL in vivo via both direct Ag presentation and cross-priming. BMDCs derived from either WT C57BL/6 (WT DCs) or MHC class I-deficient β2m-/- mice (β2m-/- DCs) were infected with rMVA-NP or control rMVA-GFP at a MOI of 10 for 6 h. The cells were washed and UV-irradiated to remove and inactivate any residual viruses. DCs were then intravenously injected into LCMV immune C57BL/6 mice 1 × (106). Five days later, NP396–404-specific CTL activity in spleens was assessed by fluorescent cellular cytotoxicity assay, as described in Methods. *: P < 0.05.

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