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Figure 8 | BMC Immunology

Figure 8

From: Dendritic Cells are preferentially targeted among hematolymphocytes by Modified Vaccinia Virus Ankara and play a key role in the induction of virus-specific T cell responses in vivo

Figure 8

DCs were required for the generation of IFN-γ-producing effector T cells following MVA infection. CD11c-DTR mice were depleted of DC by i.p. injection of DT (12ng/g body weight). Six hours after DT treatment, mice were i.p. infected with 5 × 106 PFU MVA. DT treatment was repeated on day 2 post infection. WT littermates were included as controls. At day 6 following the infection, spleens were harvested to prepare single cell suspension. (A) The efficiency of DC depletion was determined by surface cell lineage marker staining and flow cytometry. Numbers were the percentages of the gated population in total viable splenocytes. (B, C) Spleen single cell suspension (effector) was re-stimulated with MVA-infected C57BL/6 splenocytes (target) for 12 h in the presence of Brefeldin A. Following incubation, the frequencies of IFN-γ-producing T cells in the effector splenocytes were determined by intracellular cytokine staining, as described in Methods. Numbers were the percentages of IFN-γ+ cells in CD3+ T cell population. (C) The average ± SD percentages of IFN-γ+ cells for each group (n = 4). Data are representative of two independent experiments. **: P < 0.01.

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