Figure 3

Correlation between retinoid-induced IL-5 expression and T cell-associated CD38 and CD69 expression in anti-CD3, -activated PBMC. IL-5 protein levels were quantitated by ELISA in supernatants of 48 h, anti-CD3,-activated PBMC treated with ETOH or ATRA (○), 9-cis-RA (□), 13-cis-RA (●), or 4-HPR (■). The values for the level of expression (mean channel number) of CD38 and CD69 on T cells were obtained by flow cytometry of cells from the same culture. Simple regression was performed on these values as described in Materials and Methods. Data shown is average fold change calculated from three experiments.