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Figure 6 | BMC Immunology

Figure 6

From: The Retinoic Acid Receptor-α mediates human T-cell activation and Th2 cytokine and chemokine production

Figure 6

ATRA and an RAR- -α agonist upregulate and an RAR-α antagonist downregulates the expression of IL-4 and IL-5 mRNA in anti-CD3, -activated PBMC. Taqman® semi-quantitative PCR for IL-4 and IL-5 transcripts was performed using total cellular RNA of 48 h, anti-CD3,-activated PBMC treated with EtOH, ATRA (10-7 M), an RAR- α agonist, AM580 (10-7 M), or an RAR- α antagonist, RO 41–5254 (10-7 M). Values obtained for each cytokine message was normalized to that obtained for 18S rRNA in the same sample as described in "Materials and Methods". The normalized values were then expressed as a function of the ETOH control sample. The data are representative of all of the donors tested.

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