Figure 1
From: His-tag ELISA for the detection of humoral tumor-specific immunity
Capture ELISA for measurement of antibody response to his-tagged tumor-associated antigen can be developed to meet standard requirements in precision, and there is no significant difference in antibody response to the his-tagged mock vector between normal donors or cancer patients. (A) Twenty replicates of a sample positive for antibodies to IGFBP-2 were analyzed by capture ELISA to determine intra-assay precision (closed boxes) and interassay precision (open boxes). Data is expressed as IGFBP-2 IgG in ug/ml. (B) Response to the CHO cell lysate transfected with the his-tagged mock vector was assayed by capture ELISA for normal controls and cancer patients. Data is expressed as OD; bars represent mean OD for each group.