Figure 4
From: Leukotriene B4-loaded microspheres: a new therapeutic strategy to modulate cell activation
NO production (A) and PPARα expression (B) by peritoneal macrophages after 4 hours incubation in RPMI medium. Peritoneal macrophages were incubated for 4 h with LTB4 in solution (5 × 10-8 M) with or without a specific BLT1 receptor antagonist (CP105,696). Similarly, cells were incubated with unloaded or LTB4-loaded MS (1 mg/ml) in absence or presence of CP105,696. (A) The nitrites levels were quantified by Griess reaction in the supernatants of the cells. The data are expressed as means ± the SEM (n = 3); **P < 0.01, ***P < 0.001, when compared to control (medium). ##P < 0.01, LTB4-loaded MS compared to LTB4-loaded MS + CP. (B) Western blot analysis for PPARα was performed as previously described in the Materials and Methods Section. Densitometric measurements show representative data of three separated experiments (mean ± SEM). **P < 0.01, LTB4-loaded MS + CP compared to LTB4 solution + CP. ##P < 0.01, LTB4-loaded MS + CP compared to LTB4-loaded MS.