From: Regional variations in Paneth cell antimicrobial peptide expression along the mouse intestinal tract
Primers used for cloning of CRS peptides | ||
---|---|---|
Product | Sense | Antisense |
CRS | 5'aaggctctg(c/g)tcttcaagat3' 5'tg(c/g)tcttcaagatg(t/c)agccc3' | 5'ccatcctaatacgactcactatagggc3' |
CRS4C-4 | 5'gtgagtgctggactcagcc3' | 5'gattgcatttgcagctcggg3' |
CRS4C-6 | 5'cctaggaaagcctccccagt3' | 5'ttttgtcatgcaattgcacc3' |
Primers used for qRT-PCR assays | ||
CRS1C | 5'caccacccaagctccaaatacacag3' | 5'atcgtgaggaccaaaagcaaatgg3' |
CRS4C | 5'gcatggaatctgggtcaagataac3' | 5'agaaggaagagcaatcaaggctaag3' |
Crypt1 | 5'tcaagaggctgcaaaggaagagaac3' | 5'tggtctccatgttcagcgacagc3' |
Crypt4 | 5'gctgtgtctatctcctttggaggc3' | 5'cgtattccacaagtcccacgaac3' |
mLys | 5'ggctggctactatggagtcagcctg3' | 5'gcattcacagctcttggggttttg3' |
pLys | 5'gccaaggtctaacaatcgttgtgagttg3' | 5'cagtcagccagcttgacaccacg3' |
sPLA2 | 5'aggattcccccaagatgccac3' | 5'cagccgtttctgacaggagttctgg3' |
TCF-4 | 5'cggctcactccacagctcaaag3' | 5'cgagaatctggttgatggctgc3' |
NOD2 | 5'cgacatctcccacagagttgtaatcc3' | 5'ggcacctgaagttgacattttgc3' |
MMP7 | 5'ttcaagagggttagttgggggactg3' | 5'ttgtcaaagtgagcatctccgcc3' |
Reg3g | 5'cctcaggacatcttgtgtctgtgctc3' | 5'tccacctctgttgggttcatagcc3' |
Crypt4 C57BL/6 | 5'ccaggggaagatgaccaggctg3' | 5'tgcagcgacgatttctacaaaggc3' |