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Figure 4 | BMC Immunology

Figure 4

From: Comparison of human B cell activation by TLR7 and TLR9 agonists

Figure 4

Changes in mRNA and protein for CCL3 (MIP1α). Purified B cells from 3 different donors were stimulated with the indicated IRM or with CpG2006 for 2, 8 or 24 hours, and then were harvested for mRNA analysis. The fold change in gene expression at each time point, normalized to vehicle control, is shown for CCL3 (MIP1α), (Panel A). Conditioned media from the stimulated cells were collected at 8 and 24 hours after stimulation for analysis of protein production. The amount of secreted CCL3 (MIP1α) (in pg/ml) is shown in Panel B. The concentrations of the TLR agonists were: 3M-006, 5 μM; 3M-003, 1 μM; 852A, 3 μM; 3M-002, 5 μM; CpG 2006, 3 μM.

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