Figure 4
Monitoring hIL-1βP-driven luciferase expression in IL-1β-luc transgenic mice during zymosan-induced acute arthritis. (A) Female cHS4I-hIL-1βP-Luc mice (n = 3/group). were injected with zymosan (300 μg/knee) into the right rear knee joint, and vehicle (5% glucose in PBS) into the left rear knee joint. The mice were imaged at 0, 1, 3, 4, 6, 8, 24, 30, 48, 72, and 96 h following injection. The color overlay on the images represents the photons/sec/cm2/steradian (p/s/cm2/sr), as indicated by the color scale next to the images. (B) Quantification of luciferase activity (photons/sec) of the knee region by the LivingImage software. Data presented were mean ± SE.