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Figure 5 | BMC Immunology

Figure 5

From: Intragraft gene expression profile associated with the induction of tolerance

Figure 5

Validation of microarray results by real-time PCR. Selected genes (Ptger4, Tnsls10, Il2rg, Stat3, Cfh, Cxcl12, Hfe) in the early tolerance group (GalT BMT) compared to syngeneic controls were analyzed by real-time PCR to determine whether the data obtained by microarray analysis could be validated using an alternative technique. Relative cDNA expression levels were normalized with respect to GAPDH gene expression as internal control. Results are shown as the logarithmic value of mean fold-change of gene expression.

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