Table 1 TR V Region Forward primers
From: Primer sets for cloning the human repertoire of T cell Receptor Variable regions
OLIGO NAME | OLIGO SEQUENCE | TRA V GENES OPTIMALLY RECOGNIZED |
|---|---|---|
ALPHA | ||
TRAV1for | GGA CAA ARC MTT GAS CAG CC | V1-1,V1-2 |
TRAV2for | AAG GAC CAA GTG TTT CAG CC | V2 |
TRAV3for | GCT CAG TCA GTG RCY CAG CC | V3, V8-3 |
TRAV4for | GAT GCT AAG ACC ACM CAG CC | V4, V26-1, V26-2 |
TRAV5for | AGA AAA SAW STG GAG CAG AGT C | V5, V10, V22, V34, V41 |
TRAV6for | AGC CAA AAG ATA GAA CAG AA | V6 |
TRAV7for | GAA AAC CAG GTG GAG CAC AG | V7 |
TRAV8for | GCC CAG TCK GTG ASC CAG CW | V8-1, V8-2, V8-4, V8-6, V8-7 |
TRAV9for | GGA AAT TCA GTG RYC CAG AY | V9-1, V9-2 |
TRAV12for | CAG AAG GAG GTG GAG CAG RAT YC | V12-1, V12-2, V12-3 |
TRAV13for | GGA GAG ART GTG GRG CWG CA | V13-1, V13-2 |
TRAV14for | GCC CAG AAG RTW ACT CAA RC | V14/DV4, V19 |
TRAV16for | GCC CAG ASA GTS ACT CAG YC | V16, V38-1, V38-2/DV8 |
TRAV17for | AGT CAA CAG GGA GAA GAG GA | V17 |
TRAV18for | GGA GAC TCG GTT ACC CAG AC | V18 |
TRAV20for | AAA CAG GAG GTG ACG CAG AKT CC | V20, V21 |
TRAV23for | GGC CAA CAG AAG GAG AAA AG | V23/DV6 |
TRAV24for | GAG CTG AAM GTG GAA CAA AR | V24, V39 |
TRAV25for | GGA CAA CAG GTA ATG CAA AT | V25 |
TRAV27for | ACC CAG CTG CTG GAG CAG AG | V27 |
TRAV29for | AGT CAA CAG AAG AAT GAT GA | V29/DV5 |
TRAV30for | CAA CAA CCA GTG CAG AGT CC | V30 |
TRAV35for | GGT CAA CAG CTG AAT CAG AG | V35 |
TRAV36for | GAA GAC AAG GTG GTA CAA AG | V36/DV7 |
TRAV40for | AGC AAT TCA GTC AAG CAG AC | V40 |
BETA | TRBV GENES OPTIMALLY RECOGNIZED | |
TRBV2for | GAT GCT GAA GTC RCM CAG ACT CC | V2, V16, V23-1 |
TRBV3for | GAT GCW GMT GTT WCC CAG AC | V3-1, V24-1 |
TRBV4for | GAC ACT GRA GTY ACS CAG ACA CC | V4-1, V4-2, V4-3, V12-5 |
TRBV5for | GAG GCT GGA GTC ACH CAA AS | V5-1, V5-3, V9, V5-4, V5-5, V5-6, V5-7, V5-8 |
TRBV6for | GAG CCT GGW GTC ASY CAG AC | V6-1, V6-2, V6-3, V6-5, V6-6, V6-7, V6-8, V6-9,V17 |
TRBV7for | GGT GCT GGA GTY KCC CAG W | V7-1, V7-2, V7-3, V11-2, V7-4, V7-6, V7-7, V7-8, V7-9 |
TRBV10for | GAT GCT GRR ATC ACC CAG R | V6-4, V10-1, V10-2, V10-3 |
TRBV11for | GAA GCT GAA GTT GCC CAG TC | V11-1 |
TRBV13for | GAT GCT GGA GTY ATC CAG TC | V13, V12-3, V12-4 |
TRBV14for | GAA GCT GGA GTK RYT CAG T | V11-3, V14 |
TRBV15for | GAT GCC ATG GTC ATC CAG AA | V15 |
TRBV18for | AAT GCC GGC GTC ATG CAG AA | V18 |
TRBV19for | GAT GGT GGA ATC ACT CAG TC | V19 |
TRBV20for | AGT GCT GTC RTC TCT CAA MA | V20-1, V29-1 |
TRBV25for | GAA GCT GAC ATC TAC CAG AC | V25-1 |
TRBV27for | GAT GTG AAA GTR ACC CAG ARC YC | V27, V28 |
TRBV30for | ACA CTC CAG GCA CAG AGA TA | V30 |
GAMMA | TRGV GENES OPTIMALLY RECOGNIZED | |
TRGV1for | TCT TCC AAC TTG GAA GGG RG | V1, V2, V3, V4, V5, V8 |
TRGV9for | GCA GGT CAC CTA GAG CAA CC | V9 |
TRGV10for | TTA TCA AAA GTG GAG CAG TT | V10 |
TRGV11for | CTT GGG CAG TTG GAA CAA CC | V11 |
DELTA | TRDV GENES OPTIMALLY RECOGNIZED | |
TRDV1for | GCC CAG AAG GTT ACT CAA GC | V1 |
TRDV2for | GCC ATT GAG TTG GTG CCT GA | V2 |
TRDV3for | TGT GAC AAA GTA ACC CAG AG | V3 |