Skip to main content
Figure 4 | BMC Immunology

Figure 4

From: Streptococcus pneumoniae stabilizes tumor necrosis factor α mRNA through a pathway dependent on p38 MAPK but independent of Toll-like receptors

Figure 4

P38 MAPK is important for stabilization of TNF-α mRNA by S. pneumoniae. (A) RAW-TNF-AU+ cells were treated with the inhibitors SB202190 (p38 and RIP2) (5 μM) and PP2 (Src and RIP2) (500 nM) 15 min prior to addition of vehicle or S. pneumoniae (5 × 107 bacteria/ml) to the cell cultures. Total cell lysates were harvested 20 h later and CAT was measured by ELISA. The data is shown as means +/- SEM. (B) RAW264.7 cells were treated with live or heat-killed S. pneumoniae (5 × 107 bacteria/ml) for the indicated amount of time, and whole cell lysates were isolated. Phosphorylation of p38 was measured by Luminex. The data is shown as means +/- SEM. (C and D) RAW264.7 (C) and C57BL/6 macrophages (D) were incubated with a MyD88 inhibitor peptide or a control peptide for 24 h before addition of vehicle or S. pneumoniae (5 × 107 bacteria/ml). Whole cell lysates were harvested 30 min later and phosphorylation of p38 was measured by Luminex. The data is shown as means +/- SEM. Similar results were obtained in 2–3 independent experiments.

Back to article page