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Table 4 Burst activities of differentiated cells expanded in IL-stimulated endothelial supernatant.

From: Hematopoietic progenitor cells and interleukin-stimulated endothelium: expansion and differentiation of myeloid precursors

 

Control

E. coli

fMLP

PMA

IL-1β

(FBS)

52.5 ± 13.1

(593 ± 156.9)

509.5 ±

107.9 (169.1 ± 30.6)

62.7 ± 10.5

(1336 ± 320.5)

350.4 ± 95.5

(2873.4 ± 615.9)

IL-3

55.4 ± 13.2

800.7 ± 343.1

74 ± 33.1

784.3 ± 334.7

IL-6

39 ± 9.4

467.6 ± 167.31

177 ± 124

287.1 ± 104.5

  1. After culturing the cells for one week with G-CSF (100 ng/ml) and keeping them overnight in human serum, expanded cells showed a ten-fold increased burst activity in response to E. coli and PMA. Oxygen radical formation in cells from different interleukin conditions were comparable (p > 0.05). Shown are average results of mean fluorescence activities ± SE from five independent experiments. In brackets: mean results after storage in FBS-based medium (n = 10).
  2. PMA: phorbol 12-myristate 13-acetate; E. coli: Escherichia coli; fMLP: N-formyl-methionyl-leucyl-phenylalanin.